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Literature DB >> 14702326

Cloning and characterization of acetohydroxyacid synthase from Bacillus stearothermophilus.

Iris Porat¹, Michael Vinogradov, Maria Vyazmensky, Chung-Dar Lu, David M Chipman, Ahmed T Abdelal, Ze'ev Barak.

Abstract

Five genes from the ilv-leu operon from Bacillus stearothermophilus have been sequenced. Acetohydroxyacid synthase (AHAS) and its subunits were separately cloned, purified, and characterized. This thermophilic enzyme resembles AHAS III of Escherichia coli, and regulatory subunits of AHAS III complement the catalytic subunit of the AHAS of B. stearothermophilus, suggesting that AHAS III is functionally and evolutionally related to the single AHAS of gram-positive bacteria.

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Year: 2004 PMID： 14702326 PMCID： PMC305746 DOI： 10.1128/JB.186.2.570-574.2004

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

24 in total

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Journal: Methods Enzymol Date: 1988 Impact factor: 1.600

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Journal: J Bacteriol Date: 1986-06 Impact factor: 3.490

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Journal: J Bacteriol Date: 1979-02 Impact factor: 3.490

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Authors: S S Pang; R G Duggleby
Journal: Biochemistry Date: 1999-04-20 Impact factor: 3.162

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Journal: Biochim Biophys Acta Date: 1985-01-29

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Authors: C Cordes; B Möckel; L Eggeling; H Sahm
Journal: Gene Date: 1992-03-01 Impact factor: 3.688

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Authors: Z Barak; D M Chipman; N Gollop
Journal: J Bacteriol Date: 1987-08 Impact factor: 3.490

8. Cloning, sequencing and expression of the ilvBNC gene cluster from Streptomyces avermitilis.

Authors: E De Rossi; R Leva; L Gusberti; P L Manachini; G Riccardi
Journal: Gene Date: 1995-12-01 Impact factor: 3.688

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Authors: D Chipman; Z Barak; J V Schloss
Journal: Biochim Biophys Acta Date: 1998-06-29

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Authors: O Weinstock; C Sella; D M Chipman; Z Barak
Journal: J Bacteriol Date: 1992-09 Impact factor: 3.490

5 in total

1. Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit.

Authors: Janusz J Petkowski; Maksymilian Chruszcz; Matthew D Zimmerman; Heping Zheng; Tatiana Skarina; Olena Onopriyenko; Marcin T Cymborowski; Katarzyna D Koclega; Alexei Savchenko; Aled Edwards; Wladek Minor
Journal: Protein Sci Date: 2007-07 Impact factor: 6.725

2. Influence of allosteric regulators on individual steps in the reaction catalyzed by Mycobacterium tuberculosis 2-hydroxy-3-oxoadipate synthase.

Authors: Anand Balakrishnan; Frank Jordan; Carl F Nathan
Journal: J Biol Chem Date: 2013-06-11 Impact factor: 5.157