Literature DB >> 14693850

A comparison of IgG anti-rubella activity in frozen serum stored in primary gel separation tubes or secondary tubes.

V Ellis1, A Charlett, R Bendall.   

Abstract

AIMS: To determine the suitability of primary gel separation tubes for the storage of frozen sera intended for serological testing.
METHODS: Blood samples from 102 patients were collected into gel separation tubes. The sera from these samples were split between the primary gel separation tubes and conventional plastic storage tubes and frozen. A year later, the tubes were thawed and anti-rubella IgG concentrations were compared for the serum pairs using the Wilcoxon signed rank test.
RESULTS: No significant difference was detected between the two storage methods.
CONCLUSIONS: Frozen storage of serum samples in primary gel separation tubes is a practical alternative to storing separated sera in secondary containers. Adopting this practice has advantages for laboratories in reducing specimen handling and reducing errors in labelling stored samples.

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Year:  2004        PMID: 14693850      PMCID: PMC1770189          DOI: 10.1136/jcp.57.1.104

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN: 0021-9746            Impact factor:   3.411


  5 in total

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Authors:  V J Bush; M R Janu; F Bathur; A Wells; A Dasgupta
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2.  Comparison of immulite with vidas for detection of infection in a low-prevalence population of pregnant women in The Netherlands.

Authors:  F Vlaspolder; P Singer; A Smit; R J Diepersloot
Journal:  Clin Diagn Lab Immunol       Date:  2001-05

3.  Effects of handling and storage of blood on the stability of hepatitis C virus RNA: implications for NAT testing in transfusion practice.

Authors:  P R Grant; A Kitchen; J A Barbara; P Hewitt; C M Sims; J A Garson; R S Tedder
Journal:  Vox Sang       Date:  2000       Impact factor: 2.144

4.  Serological testing in a microbiology laboratory of specimens from patients with suspected infectious disease.

Authors:  D J Waghorn
Journal:  J Clin Pathol       Date:  1995-04       Impact factor: 3.411

5.  Suitability of collection tubes with separator gels for collecting and storing blood samples for therapeutic drug monitoring (TDM).

Authors:  J Karppi; K K Akerman; M Parviainen
Journal:  Clin Chem Lab Med       Date:  2000-04       Impact factor: 3.694

  5 in total
  3 in total

1.  Limitations of the Wilcoxon matched pairs signed ranks test for comparison studies.

Authors:  P J Twomey; A Viljoen
Journal:  J Clin Pathol       Date:  2004-07       Impact factor: 3.411

2.  Collection tubes with or without gel separator did not interfere with detection of rubella virus antibodies IgM and IgG.

Authors:  L C Oliveira; K H Kawasato; M S Otta; L P Lima; T S Okay
Journal:  J Clin Lab Anal       Date:  2007       Impact factor: 2.352

Review 3.  Bio-banking in microbiology: from sample collection to epidemiology, diagnosis and research.

Authors:  Paolo De Paoli
Journal:  FEMS Microbiol Rev       Date:  2005-02-26       Impact factor: 16.408

  3 in total

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