| Literature DB >> 14690245 |
Guoping Ren1, Zong Lin, Chen-lu Tsou, Chih-chen Wang.
Abstract
The effects of crowding agents, polyethylene glycol (PEG 20K), Dextran 70, and bovine serum albumin, on the denaturation of homotetrameric D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) in 0.5 M guanidine hydrochloride and the reactivation of the fully denatured enzyme have been examined quantitatively. Increasing the concentration of PEG 20K to 225 mg/ml decreases the rate constant of slow phase of GAPDH inactivation to 5% but with no change for the fast phase. Chaperone GroEL assists GAPDH refolding greatly and shows even higher efficiency under crowding condition. Crowding mainly affects refolding steps after the formation of the dimeric folding intermediate.Entities:
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Year: 2003 PMID： 14690245 DOI： 10.1023/b:jopc.0000005458.08802.11
Source DB: PubMed Journal: J Protein Chem ISSN： 0277-8033