Literature DB >> 14646118

Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals.

Todd Holyoak1, Timothy D Fenn, Mark A Wilson, Aaron G Moulin, Dagmar Ringe, Gregory A Petsko.   

Abstract

Collection of quality data on high-energy synchrotron sources requires the selection of a cryoprotectant that allows protein crystals to be cryocooled without damage to the crystal and with suppression of ice formation. The use of sodium malonate as a versatile cryoprotectant for salt-grown protein crystals is presented here. In addition to its useful cryoprotectant properties, sodium malonate can also function as a versatile stabilizing solution that allows the manipulation, derivatization and ligand soaking of crystals grown from salt that may not be possible in the crystal mother liquor.

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Year:  2003        PMID： 14646118     DOI： 10.1107/s0907444903021784

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  30 in total

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5.  Ellman's reagent in promoting crystallization and structure determination of Anabaena CcbP.

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