Literature DB >> 14617662

Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.

Masayuki Kagawa1, Zui Fujimoto, Mitsuru Momma, Kenji Takase, Hiroshi Mizuno.   

Abstract

The crystal structure of Bacillus subtilis alpha-amylase, in complex with the pseudotetrasaccharide inhibitor acarbose, revealed an hexasaccharide in the active site as a result of transglycosylation. After comparison with the known structure of the catalytic-site mutant complexed with the native substrate maltopentaose, it is suggested that the present structure represents a mimic intermediate in the initial stage of the catalytic process.

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Year:  2003        PMID： 14617662      PMCID： PMC262715          DOI： 10.1128/JB.185.23.6981-6984.2003

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

1.  Site-directed mutagenesis of active site residues in Bacillus subtilis alpha-amylase.

Authors:  K Takase; T Matsumoto; H Mizuno; K Yamane
Journal:  Biochim Biophys Acta       Date:  1992-04-17

2.  An extensively modified version of MolScript that includes greatly enhanced coloring capabilities.

Authors:  R M Esnouf
Journal:  J Mol Graph Model       Date:  1997-04       Impact factor: 2.518

3.  Enzyme-catalyzed condensation reaction in a mammalian alpha-amylase. High-resolution structural analysis of an enzyme-inhibitor complex.

Authors:  M Qian; V Nahoum; J Bonicel; H Bischoff; B Henrissat; F Payan
Journal:  Biochemistry       Date:  2001-06-26       Impact factor: 3.162

4.  Crystallographic evidence of a transglycosylation reaction: ternary complexes of a psychrophilic alpha-amylase.

Authors:  Nushin Aghajari; Michel Roth; Richard Haser
Journal:  Biochemistry       Date:  2002-04-02       Impact factor: 3.162

5.  Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 angstrom resolution. Implications for product specificity.

Authors:  B Strokopytov; R M Knegtel; D Penninga; H J Rozeboom; K H Kalk; L Dijkhuizen; B W Dijkstra
Journal:  Biochemistry       Date:  1996-04-02       Impact factor: 3.162

6.  Structural analysis of a chimeric bacterial alpha-amylase. High-resolution analysis of native and ligand complexes.

Authors:  A M Brzozowski; D M Lawson; J P Turkenburg; H Bisgaard-Frantzen; A Svendsen; T V Borchert; Z Dauter; K S Wilson; G J Davies
Journal:  Biochemistry       Date:  2000-08-08       Impact factor: 3.162

7.  The active center of a mammalian alpha-amylase. Structure of the complex of a pancreatic alpha-amylase with a carbohydrate inhibitor refined to 2.2-A resolution.

Authors:  M Qian; R Haser; G Buisson; E Duée; F Payan
Journal:  Biochemistry       Date:  1994-05-24       Impact factor: 3.162

8.  Interaction of catalytic-site mutants of Bacillus subtilis alpha-amylase with substrates and acarbose.

Authors:  K Takase
Journal:  Biochim Biophys Acta       Date:  1992-08-21

9.  NH2-terminal processing of Bacillus subtilis alpha-amylase.

Authors:  K Takase; H Mizuno; K Yamane
Journal:  J Biol Chem       Date:  1988-08-15       Impact factor: 5.157

10.  X-ray structure of cyclodextrin glycosyltransferase complexed with acarbose. Implications for the catalytic mechanism of glycosidases.

Authors:  B Strokopytov; D Penninga; H J Rozeboom; K H Kalk; L Dijkhuizen; B W Dijkstra
Journal:  Biochemistry       Date:  1995-02-21       Impact factor: 3.162
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  8 in total

1.  New insight in the structural features of haloadaptation in α-amylases from halophilic Archaea following homology modeling strategy: folded and stable conformation maintained through low hydrophobicity and highly negative charged surface.

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Journal:  J Comput Aided Mol Des       Date:  2014-05-28       Impact factor: 3.686

2.  Crystal structure of thermally stable homodimeric cytochrome c'-β from Thermus thermophilus.

Authors:  Taisuke Yoshimi; Sotaro Fujii; Hiroya Oki; Takeshi Igawa; Hannah R Adams; Kengo Ueda; Kazuki Kawahara; Tadayasu Ohkubo; Michael A Hough; Yoshihiro Sambongi
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2022-05-27       Impact factor: 1.072

3.  Sequence and structural investigation of a novel psychrophilic α-amylase from Glaciozyma antarctica PI12 for cold-adaptation analysis.

Authors:  Aizi Nor Mazila Ramli; Mohd Akmal Azhar; Mohd Shahir Shamsir; Amir Rabu; Abdul Munir Abdul Murad; Nor Muhammad Mahadi; Rosli Md Illias
Journal:  J Mol Model       Date:  2013-05-18       Impact factor: 1.810

4.  Ameliorative effect of rubiadin-loaded nanocarriers in STZ-NA-induced diabetic nephropathy in rats: formulation optimization, molecular docking, and in vivo biological evaluation.

Authors:  Mohd Mujeeb; Abdul Ahad; Mohd Aqil; Waseem Ahmad Siddiqui; Abul Kalam Najmi; Mymoona Akhtar; Apeksha Shrivastava; Abdul Qadir; Thasleem Moolakkadath
Journal:  Drug Deliv Transl Res       Date:  2021-05-19       Impact factor: 4.617

5.  Functional and cooperative stabilization of a two-metal (Ca, Zn) center in α-amylase derived from Flavobacteriaceae species.

Authors:  Huijia Yin; Zhou Yang; Xinyu Nie; Shannan Li; Xuyang Sun; Chao Gao; Zenghang Wang; Guangming Zhou; Ping Xu; Chunyu Yang
Journal:  Sci Rep       Date:  2017-12-20       Impact factor: 4.379

6.  Molecular Engineering of the Geobacillus stearothermophilus α-Amylase and Cel5E from Chlostridium thermocellim; In Silico Approach.

Authors:  Ibrahim Torktaz; Jafar Hemmat; Ali Asghar Karkhane; Garshasb Rigi; Amin Rostami; Jafar Khezri; Reza Behroozi
Journal:  Iran J Biotechnol       Date:  2018-08-11       Impact factor: 1.671

7.  D-arginine Enhances the Effect of Alpha-Amylase on Disassembling Actinomyces viscosus Biofilm.

Authors:  Baosheng Li; Qing Cai; Zixuan Wang; Shuwei Qiao; Yanzhen Ou; Rui Ma; Chuanfu Luo; Weiyan Meng
Journal:  Front Bioeng Biotechnol       Date:  2022-03-03

8.  PLG-007 and Its Active Component Galactomannan-α Competitively Inhibit Enzymes That Hydrolyze Glucose Polymers.

Authors:  Michelle C Miller; Aurelio J Dregni; David Platt; Kevin H Mayo
Journal:  Int J Mol Sci       Date:  2022-07-13       Impact factor: 6.208
  8 in total

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