Literature DB >> 14614744

Development of a dipstick dye immunoassay for diagnosing hydatidosis.

Younes Sbihi1, José R Gil, Pedro A Alvarez, Antonio Orduña, Antonio Rodríguez-Torres, Antonio Osuna.   

Abstract

We have developed a kit to diagnose hydatidosis, based on the detection of specific antibodies. This disease, caused by larvae of the cestode Echinococcus granulosus, is a zoonosis commonly found throughout the world. The diagnostic kit discriminates between positive and negative cases in a way that is easily interpreted, i.e., positive cases form a colored band on an inert substrate. The results show test sensitivity to be some 94.87% and specificity 85.71%, which is comparable to those of the ELISA technique. The high sensitivity and specificity of this test affords the great advantage of speed in diagnosing this parasitosis. No other equipment or procedure is required. Copyright 2003 Wiley-Liss, Inc.

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Year:  2003        PMID: 14614744      PMCID: PMC6808177          DOI: 10.1002/jcla.10097

Source DB:  PubMed          Journal:  J Clin Lab Anal        ISSN: 0887-8013            Impact factor:   2.352


  11 in total

1.  Western blot applied to the diagnosis and post-treatment monitoring of human hydatidosis.

Authors:  O Doiz; R Benito; Y Sbihi; A Osuna; A Clavel; R Gómez-Lus
Journal:  Diagn Microbiol Infect Dis       Date:  2001-11       Impact factor: 2.803

2.  Echinococcus multilocularis in the mouse: the in vitro protoscolicidal activity of peritoneal macrophages.

Authors:  R W Baron; C E Tanner
Journal:  Int J Parasitol       Date:  1977-12       Impact factor: 3.981

3.  [Localization of hydatidosis in the central nervous system].

Authors:  P M Schantz
Journal:  Bol Oficina Sanit Panam       Date:  1972-09

4.  Electron microscopic localization of glutamate dehydrogenase in rat liver mitochondria by an immunogold procedure and monoclonal and polyclonal antibodies.

Authors:  E Knecht; A Martinez-Ramon; S Grisolia
Journal:  J Histochem Cytochem       Date:  1986-07       Impact factor: 2.479

5.  Evaluation of purified lipoprotein antigens of Echinococcus granulosus in the immunodiagnosis of human infection.

Authors:  J F Williams; M V Pérez Esandi; R Oriol
Journal:  Am J Trop Med Hyg       Date:  1971-07       Impact factor: 2.345

6.  Cystolytic events and the possible role of germinal cells in metastasis in chronic alveolar hydatidosis.

Authors:  Z Ali-Khan; R Siboo; M Gomersall; M Faucher
Journal:  Ann Trop Med Parasitol       Date:  1983-10

7.  Evaluation of six serological tests in diagnosis and postoperative control of pulmonary hydatid disease patients.

Authors:  M P Zarzosa; A Orduña Domingo; P Gutiérrez; P Alonso; M Cuervo; A Prado; M A Bratos; M García-Yuste; G Ramos; A Rodríguez Torres
Journal:  Diagn Microbiol Infect Dis       Date:  1999-12       Impact factor: 2.803

8.  Comparative sensitivity of six serological tests and diagnostic value of ELISA using purified antigen in hydatidosis.

Authors:  Y Sbihi; A Rmiqui; M N Rodriguez-Cabezas; A Orduña; A Rodriguez-Torres; A Osuna
Journal:  J Clin Lab Anal       Date:  2001       Impact factor: 2.352

9.  Detection of antibodies against Echinococcus granulosus major antigens and their subunits by immunoblotting.

Authors:  A Siracusano; S Ioppolo; S Notargiacomo; E Ortona; R Riganó; A Teggi; F De Rosa; G Vicari
Journal:  Trans R Soc Trop Med Hyg       Date:  1991 Mar-Apr       Impact factor: 2.184

10.  Serologic recognition of hydatid cyst antigens using different purification methods.

Authors:  Y Sbihi; D Janssen; A Osuna
Journal:  Diagn Microbiol Infect Dis       Date:  1996-04       Impact factor: 2.803

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  3 in total

1.  A Comparative Evaluation of Four Different Immunoassays in the Diagnosis of Cystic Echinococcosis Using a Crude and Purified Hydatid Cyst Fluid Antigen.

Authors:  P Vinayaka Siddartha; A Jagadeesh Babu; T Madhava Rao; V Chengalva Rayulu; C S Swetha
Journal:  Acta Parasitol       Date:  2022-09-20       Impact factor: 1.534

2.  Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test.

Authors:  Rahmah Noordin; Sam Khanbabaie; Muhammad Hafiznur Yunus; Hanspeter Marti; Beatrice Nickel; Majid Fasihi Harandi; Saeid Nasibi
Journal:  Iran J Parasitol       Date:  2020 Jul-Sep       Impact factor: 1.012

3.  Lateral flow test using Echinococcus granulosus native antigen B and comparison of IgG and IgG4 dipsticks for detection of human cystic echinococcosis.

Authors:  Akbar Khalilpour; Seyed Mahmoud Sadjjadi; Zohreh Kazemi Moghadam; Muhammad Hafiznur Yunus; Nor Dyana Zakaria; Sabariah Osman; Rahmah Noordin
Journal:  Am J Trop Med Hyg       Date:  2014-09-08       Impact factor: 2.345

  3 in total

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