Literature DB >> 14611502

Exponential DNA replication by laminar convection.

Dieter Braun1, Noel L Goddard, Albert Libchaber.   

Abstract

It is shown that laminar thermal convection can drive a chain reaction of DNA replication. The convection is triggered by a constant horizontal temperature gradient, moving molecules along stationary paths between hot and cold regions. This implements the temperature cycling for the classical polymerase chain reaction (PCR). The amplification is shown to be exponential and reaches 100,000-fold gains within 25 min. Besides direct applications, the mechanism might have implications for the molecular evolution of life.

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Year:  2003        PMID: 14611502     DOI: 10.1103/PhysRevLett.91.158103

Source DB:  PubMed          Journal:  Phys Rev Lett        ISSN: 0031-9007            Impact factor:   9.161


  25 in total

1.  Extreme accumulation of nucleotides in simulated hydrothermal pore systems.

Authors:  Philipp Baaske; Franz M Weinert; Stefan Duhr; Kono H Lemke; Michael J Russell; Dieter Braun
Journal:  Proc Natl Acad Sci U S A       Date:  2007-05-09       Impact factor: 11.205

2.  Protein-mediated selective enclosure of early replicators inside of membranous vesicles: first step towards cell membranes.

Authors:  Tiina Laiterä; Kirsi Lehto
Journal:  Orig Life Evol Biosph       Date:  2009-12       Impact factor: 1.950

3.  Heat flux across an open pore enables the continuous replication and selection of oligonucleotides towards increasing length.

Authors:  Moritz Kreysing; Lorenz Keil; Simon Lanzmich; Dieter Braun
Journal:  Nat Chem       Date:  2015-01-26       Impact factor: 24.427

4.  Characterization and analysis of real-time capillary convective PCR toward commercialization.

Authors:  Xianbo Qiu; Shiyin Zhang; Lanju Mei; Di Wu; Qi Guo; Ke Li; Shengxiang Ge; Xiangzhong Ye; Ningshao Xia; Michael G Mauk
Journal:  Biomicrofluidics       Date:  2017-03-03       Impact factor: 2.800

5.  Highly multiplexed rapid DNA detection with single-nucleotide specificity via convective PCR in a portable device.

Authors:  Dmitriy Khodakov; Jiaming Li; Jinny X Zhang; David Yu Zhang
Journal:  Nat Biomed Eng       Date:  2021-07-01       Impact factor: 25.671

Review 6.  Opto-thermal diffusiophoresis of soft biological matter: from physical principle to molecular manipulation.

Authors:  Tatsuya Fukuyama; Yusuke T Maeda
Journal:  Biophys Rev       Date:  2020-04-17

7.  Development of an artificial cell, from self-organization to computation and self-reproduction.

Authors:  Vincent Noireaux; Yusuke T Maeda; Albert Libchaber
Journal:  Proc Natl Acad Sci U S A       Date:  2011-02-11       Impact factor: 11.205

8.  Effects of long DNA folding and small RNA stem-loop in thermophoresis.

Authors:  Yusuke T Maeda; Tsvi Tlusty; Albert Libchaber
Journal:  Proc Natl Acad Sci U S A       Date:  2012-10-15       Impact factor: 11.205

9.  Thermostability of model protocell membranes.

Authors:  Sheref S Mansy; Jack W Szostak
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-03       Impact factor: 11.205

10.  Petri dish PCR: laser-heated reactions in nanoliter droplet arrays.

Authors:  Hanyoup Kim; Siarhei Vishniakou; Gregory W Faris
Journal:  Lab Chip       Date:  2009-01-19       Impact factor: 6.799

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