| Literature DB >> 14607426 |
Cornelly Williams1, Omar A Espinosa, Hector Montenegro, Luis Cubilla, Todd L Capson, Eduardo Ortega-Barría, Luz I Romero.
Abstract
A colorimetric method for measuring the viability of Leishmania promastigotes is described that is based on the reduction of the tetrazolium salt, XTT, to a water-soluble formazan. Values obtained by the XTT method correlated well with parasite number (r=0.965) and with methods that rely upon the reduction of MTT or MTS (r=0.96 and 0.97, respectively). The IC(50) values obtained by XTT method with amphotericin-B, miltefosine and ketoconazole were similar to those previously reported by other methods. The XTT method proved to be a reliable and convenient method for the screening of methanolic extracts from 1059 plants and was used for the bioassay-guided fractionation of the alkaloid aegeline from Sarcorhachis naranjoana.Entities:
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Year: 2003 PMID： 14607426 DOI： 10.1016/j.mimet.2003.08.013
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363