Literature DB >> 14605937

Preliminary comparison of three LightCycler PCR assays for the detection of herpes simplex virus in swab specimens.

D M Whiley1, M W Syrmis, I M Mackay, T P Sloots.   

Abstract

Three Herpes Simplex Virus LightCycler polymerase chain reaction assays were compared for the detection of herpes simplex virus in 48 swab specimens. The assays comprised of one in-house assay and two commercial kits: the Artus HSV LC RealArt PCR kit and the Roche LightCycler HSV 1/2 Detection kit. On the whole, the three assays had comparable sensitivities. However, differentiation of herpes simplex virus types 1 and 2 by melting curve analysis was problematic in all assays. Overall, the results highlight the limitations of typing herpes simplex virus by melting curve analysis.

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Year:  2003        PMID： 14605937     DOI： 10.1007/s10096-003-1031-2

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  10 in total

1.  Fast and type-specific analysis of herpes simplex virus types 1 and 2 by rapid PCR and fluorescence melting-curve-analysis.

Authors:  G Schalasta; A Arents; M Schmid; R W Braun; G Enders
Journal:  Infection       Date:  2000 Mar-Apr       Impact factor: 3.553

2.  Detection of cytomegalovirus DNA in human specimens by LightCycler PCR: melting point analysis is mandatory to detect virus strains with point mutations in the target sequence of the hybridization probes.

Authors:  L Schaade; P Kockelkorn; K Ritter; M Kleines
Journal:  J Clin Microbiol       Date:  2001-10       Impact factor: 5.948

3.  Quantitative PCR-ELAHA for the determination of retroviral vector transduction efficiency.

Authors:  I M Mackay; P Metharom; T P Sloots; M Q Wei
Journal:  Mol Ther       Date:  2001-05       Impact factor: 11.454

4.  A quantification of human cells using an ERV-3 real time PCR assay.

Authors:  C C Yuan; W Miley; D Waters
Journal:  J Virol Methods       Date:  2001-02       Impact factor: 2.014

5.  Diagnosis of herpes simplex virus infections in the clinical laboratory by LightCycler PCR.

Authors:  M J Espy; J R Uhl; P S Mitchell; J N Thorvilson; K A Svien; A D Wold; T F Smith
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

6.  A convenient approach to the generation of multiple internal control DNA for a panel of real-time PCR assays.

Authors:  Markus Stöcher; Victoria Leb; Jörg Berg
Journal:  J Virol Methods       Date:  2003-03       Impact factor: 2.014

7.  Aseptic meningitis and encephalitis: the role of PCR in the diagnostic laboratory.

Authors:  S J Read; K J Jeffery; C R Bangham
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

8.  Detection of herpes simplex virus (HSV) genome using polymerase chain reaction (PCR) in clinical samples comparison of PCR with standard laboratory methods for the detection of HSV.

Authors:  H N Madhavan; K Priya; A R Anand; K L Therese
Journal:  J Clin Virol       Date:  1999-10       Impact factor: 3.168

9.  Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples.

Authors:  A J Ryncarz; J Goddard; A Wald; M L Huang; B Roizman; L Corey
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

10.  Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture.

Authors:  Julie Burrows; Andreas Nitsche; Belinda Bayly; Elise Walker; Geoff Higgins; TuckWeng Kok
Journal:  BMC Microbiol       Date:  2002-06-09       Impact factor: 3.605
  10 in total
  6 in total

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Authors:  Hatim T Allawi; Haijing Li; Tamara Sander; Azamat Aslanukov; Victor I Lyamichev; Amondrea Blackman; Slava Elagin; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2006-09       Impact factor: 5.948

2.  HSV-1 DNA in tears and saliva of normal adults.

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Journal:  Invest Ophthalmol Vis Sci       Date:  2005-01       Impact factor: 4.799

3.  The diagnosis of genital herpes - beyond culture: An evidence-based guide for the utilization of polymerase chain reaction and herpes simplex virus type-specific serology.

Authors:  S Ratnam; A Severini; G Zahariadis; M Petric; B Romanowski
Journal:  Can J Infect Dis Med Microbiol       Date:  2007-07       Impact factor: 2.471

4.  Bayesian Evaluation of Solana HSV 1+2/VZV Assay Compared to Viral Culture and Commercial PCR Assay for Cutaneous or Mucocutaneous Specimens.

Authors:  Christine Arsenault; Félix Camirand Lemyre; Philippe Martin; Simon Lévesque
Journal:  J Clin Microbiol       Date:  2020-02-24       Impact factor: 5.948

5.  Comparison of different polymerase chain reaction methods for detection of herpes simplex virus types 1 and 2 encephalitis.

Authors:  I Altuglu; A Zeytinoglu; H Sirin; N Yuceyar; S Erensoy
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2006-10       Impact factor: 5.103

6.  Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction.

Authors:  Mark W Pandori; John Lei; Ernest H Wong; Jeffrey Klausner; Sally Liska
Journal:  BMC Infect Dis       Date:  2006-06-24       Impact factor: 3.090
  6 in total

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