| Literature DB >> 14605208 |
L Giot1, J S Bader, C Brouwer, A Chaudhuri, B Kuang, Y Li, Y L Hao, C E Ooi, B Godwin, E Vitols, G Vijayadamodar, P Pochart, H Machineni, M Welsh, Y Kong, B Zerhusen, R Malcolm, Z Varrone, A Collis, M Minto, S Burgess, L McDaniel, E Stimpson, F Spriggs, J Williams, K Neurath, N Ioime, M Agee, E Voss, K Furtak, R Renzulli, N Aanensen, S Carrolla, E Bickelhaupt, Y Lazovatsky, A DaSilva, J Zhong, C A Stanyon, R L Finley, K P White, M Braverman, T Jarvie, S Gold, M Leach, J Knight, R A Shimkets, M P McKenna, J Chant, J M Rothberg.
Abstract
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.Entities:
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Year: 2003 PMID： 14605208 DOI： 10.1126/science.1090289
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728