Literature DB >> 14594850

In vivo effect of mutations at the PRPP binding site of the Bacillus subtilis purine repressor.

Pekka Rappu1, Terhi Pullinen, Pekka Mäntsälä.   

Abstract

The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR.

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Year:  2003        PMID： 14594850      PMCID： PMC262106          DOI： 10.1128/JB.185.22.6728-6731.2003

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  11 in total

1.  A role for a highly conserved protein of unknown function in regulation of Bacillus subtilis purA by the purine repressor.

Authors:  P Rappu; B S Shin; H Zalkin; P Mäntsälä
Journal:  J Bacteriol       Date:  1999-06       Impact factor: 3.490

Review 2.  The PRT protein family.

Authors:  S C Sinha; J L Smith
Journal:  Curr Opin Struct Biol       Date:  2001-12       Impact factor: 6.809

3.  Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools.

Authors:  H H Saxild; P Nygaard
Journal:  J Gen Microbiol       Date:  1991-10

4.  Interaction of Bacillus subtilis purine repressor with DNA.

Authors:  B S Shin; A Stein; H Zalkin
Journal:  J Bacteriol       Date:  1997-12       Impact factor: 3.490

5.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

6.  A neomycin resistance gene cassette selectable in a single copy state in the Bacillus subtilis chromosome.

Authors:  M Itaya; K Kondo; T Tanaka
Journal:  Nucleic Acids Res       Date:  1989-06-12       Impact factor: 16.971

7.  Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.

Authors:  H H Saxild; K Brunstedt; K I Nielsen; H Jarmer; P Nygaard
Journal:  J Bacteriol       Date:  2001-11       Impact factor: 3.490

8.  Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis.

Authors:  D J Ebbole; H Zalkin
Journal:  J Biol Chem       Date:  1987-06-15       Impact factor: 5.157

9.  Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.

Authors:  M Weng; H Zalkin
Journal:  Curr Microbiol       Date:  2000-07       Impact factor: 2.188

10.  Identification of the Bacillus subtilis pur operon repressor.

Authors:  M Weng; P L Nagy; H Zalkin
Journal:  Proc Natl Acad Sci U S A       Date:  1995-08-01       Impact factor: 11.205
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  2 in total

1.  Mutational analysis of the Bacillus subtilis purA operator site.

Authors:  Pekka Rappu; Mari Leppihalme; Pekka Mäntsälä
Journal:  Curr Microbiol       Date:  2005-09-16       Impact factor: 2.188

2.  The nucleotide messenger (p)ppGpp is an anti-inducer of the purine synthesis transcription regulator PurR in Bacillus.

Authors:  Brent W Anderson; Maria A Schumacher; Jin Yang; Asan Turdiev; Husan Turdiev; Jeremy W Schroeder; Qixiang He; Vincent T Lee; Richard G Brennan; Jue D Wang
Journal:  Nucleic Acids Res       Date:  2022-01-25       Impact factor: 16.971
  2 in total

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