Literature DB >> 1459406

Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000.

C Guilhot1, B Gicquel, C Martín.   

Abstract

Two plasmids were isolated as thermosensitive replicons following in vitro mutagenesis of pB4, a pAL5000 derivative mycobacteria/Escherichia coli shuttle plasmid. Plasmids pCG59 and pCG63 replicate at 30 degrees C but not at 39 degrees C. This will allow their utilisation for transposon delivery, site-specific integration, or allele exchange.

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Year:  1992        PMID： 1459406     DOI： 10.1016/0378-1097(92)90152-e

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  19 in total

Review 1.  Mycobacteria: bugs and bugbears (two steps forward and one step back).

Authors:  T Parish; N G Stoker
Journal:  Mol Biotechnol       Date:  1999-12-15       Impact factor: 2.695

2.  Establishment of Tn5096-based transposon mutagenesis in Gordonia polyisoprenivorans.

Authors:  Quyen Banh; Matthias Arenskötter; Alexander Steinbüchel
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

3.  ACCase 6 is the essential acetyl-CoA carboxylase involved in fatty acid and mycolic acid biosynthesis in mycobacteria.

Authors:  Daniel G Kurth; Gabriela M Gago; Agustina de la Iglesia; Bernardo Bazet Lyonnet; Ting-Wan Lin; Héctor R Morbidoni; Shiou-Chuan Tsai; Hugo Gramajo
Journal:  Microbiology (Reading)       Date:  2009-05-07       Impact factor: 2.777

4.  Isolation by genetic labeling of a new mycobacterial plasmid, pJAZ38, from Mycobacterium fortuitum.

Authors:  J A Gavigan; J A Aínsa; E Pérez; I Otal; C Martín
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

5.  Defining mycobacteria: Shared and specific genome features for different lifestyles.

Authors:  Varalakshmi D Vissa; Rama Murthy Sakamuri; Wei Li; Patrick J Brennan
Journal:  Indian J Microbiol       Date:  2009-02-05       Impact factor: 2.461

6.  AccD6, a key carboxyltransferase essential for mycolic acid synthesis in Mycobacterium tuberculosis, is dispensable in a nonpathogenic strain.

Authors:  Jakub Pawelczyk; Anna Brzostek; Laurent Kremer; Bozena Dziadek; Anna Rumijowska-Galewicz; Marta Fiolka; Jaroslaw Dziadek
Journal:  J Bacteriol       Date:  2011-10-07       Impact factor: 3.490

7.  Phenotypic mutants of the intracellular actinomycete Rhodococcus equi created by in vivo Himar1 transposon mutagenesis.

Authors:  Joseph Ashour; Mary K Hondalus
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

8.  Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria.

Authors:  V Pelicic; J M Reyrat; B Gicquel
Journal:  J Bacteriol       Date:  1996-02       Impact factor: 3.490

9.  In vivo growth characteristics of leucine and methionine auxotrophic mutants of Mycobacterium bovis BCG generated by transposon mutagenesis.

Authors:  R A McAdam; T R Weisbrod; J Martin; J D Scuderi; A M Brown; J D Cirillo; B R Bloom; W R Jacobs
Journal:  Infect Immun       Date:  1995-03       Impact factor: 3.441

10.  Escherichia coli-mycobacteria shuttle vectors for operon and gene fusions to lacZ: the pJEM series.

Authors:  J Timm; E M Lim; B Gicquel
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490
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