| Literature DB >> 14592415 |
Yuika Ikeda1, Sumiko Abe-Dohmae, Youichi Munehira, Ryo Aoki, Shinobu Kawamoto, Akiko Furuya, Kenya Shitara, Teruo Amachi, Noriyuki Kioka, Michinori Matsuo, Shinji Yokoyama, Kazumitsu Ueda.
Abstract
ABCA7 is expressed predominantly in myelo-lymphatic tissues or reticuloendothelial cells. Physiological role and function of this protein are not fully understood. We isolated the full-length cDNA (type I) and a splicing variant cDNA (type II) of human ABCA7, and developed monoclonal antibodies against extracellular domain (ECD)1 of ABCA7. RT-PCR experiments suggested that human ABCA7 gene produced the type II mRNA in a tissue-specific manner. Immunostaining revealed that the type I ABCA7, expressed in HEK293 cells, was localized to the plasma membrane and ECD1 was exposed to the extracellular space as was the case for ABCA1. HEK293 cells expressing type I ABCA7 showed apoA-I-dependent cholesterol and phospholipid release. In contrast, type II ABCA7 appeared to be localized mainly in endoplasmic reticulum and did not show apoA-I-dependent cholesterol and phospholipid release. Alternative splicing could be involved in the post-transcriptional regulation of the expression and function of human ABCA7.Entities:
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Year: 2003 PMID： 14592415 DOI： 10.1016/j.bbrc.2003.10.002
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575