BACKGROUND: Cytokines secreted in human milk might play important roles in newborn health and in the development of infant immune responses. We investigated the relationship of the concentration and dose of cytokines in human milk to infant wheeze at 1 year of age. OBJECTIVE: Our objective was to test whether the cytokines in milk could account for some of the apparent protective effect of breast-feeding against wheeze in the first year of life. METHODS: Data on breast-feeding and infant wheeze were collected prospectively from birth to 1 year from 243 mothers participating in the Infant Immune Study in Tucson, Arizona. Breast milk samples obtained at a mean age of 11 days postpartum were assayed by means of ELISA for concentrations of TGF-beta1, IL-10, TNF-alpha, and the soluble form of CD14. The dose of each cytokine was assessed for a relationship with wheeze in bivariate and logistic regression analyses. RESULTS: Increasing duration of breast-feeding was significantly associated with a decreased prevalence of wheeze (P =.039). There was wide variability in levels of each cytokine in milk, as well as variability between women in the amount of each cytokine produced. There was a significant inverse association between the dose of TGF-beta1 received through milk with the percentage of wheeze (P =.017), and the relationship was linear (P =.006). None of the other cytokines showed a linear relationship with wheeze. In multivariate analyses the risk of wheeze was significantly decreased (odds ratio, 0.22; 95% CI 0.05-0.89; P =.034) with increasing TGF-beta1 dose (long breast-feeding and medium-high TGF-beta1 level compared with short breast-feeding and low TGF-beta. CONCLUSION: This analysis shows that the dose of TGF-beta1 received from milk has a significant relationship with infant wheeze, which might account for at least some of the protective effect of breast-feeding against wheeze.
BACKGROUND: Cytokines secreted in human milk might play important roles in newborn health and in the development of infant immune responses. We investigated the relationship of the concentration and dose of cytokines in human milk to infant wheeze at 1 year of age. OBJECTIVE: Our objective was to test whether the cytokines in milk could account for some of the apparent protective effect of breast-feeding against wheeze in the first year of life. METHODS: Data on breast-feeding and infant wheeze were collected prospectively from birth to 1 year from 243 mothers participating in the Infant Immune Study in Tucson, Arizona. Breast milk samples obtained at a mean age of 11 days postpartum were assayed by means of ELISA for concentrations of TGF-beta1, IL-10, TNF-alpha, and the soluble form of CD14. The dose of each cytokine was assessed for a relationship with wheeze in bivariate and logistic regression analyses. RESULTS: Increasing duration of breast-feeding was significantly associated with a decreased prevalence of wheeze (P =.039). There was wide variability in levels of each cytokine in milk, as well as variability between women in the amount of each cytokine produced. There was a significant inverse association between the dose of TGF-beta1 received through milk with the percentage of wheeze (P =.017), and the relationship was linear (P =.006). None of the other cytokines showed a linear relationship with wheeze. In multivariate analyses the risk of wheeze was significantly decreased (odds ratio, 0.22; 95% CI 0.05-0.89; P =.034) with increasing TGF-beta1 dose (long breast-feeding and medium-high TGF-beta1 level compared with short breast-feeding and low TGF-beta. CONCLUSION: This analysis shows that the dose of TGF-beta1 received from milk has a significant relationship with infant wheeze, which might account for at least some of the protective effect of breast-feeding against wheeze.
Authors: Haili Xu; Timothy Radabaugh; Zhenqiang Lu; Michael Galligan; Dean Billheimer; Donata Vercelli; Anne L Wright; Terrence J Monks; Marilyn Halonen; Serrine S Lau Journal: Pediatr Allergy Immunol Date: 2016-09-12 Impact factor: 6.377
Authors: Pamela A Frischmeyer-Guerrerio; Anthony L Guerrerio; Gretchen Oswald; Kristin Chichester; Loretha Myers; Marc K Halushka; Maria Oliva-Hemker; Robert A Wood; Harry C Dietz Journal: Sci Transl Med Date: 2013-07-24 Impact factor: 17.956
Authors: Avery DeVries; Gabriela Wlasiuk; Susan J Miller; Anthony Bosco; Debra A Stern; I Carla Lohman; Janet Rothers; Anya C Jones; Jessie Nicodemus-Johnson; Monica M Vasquez; John A Curtin; Angela Simpson; Adnan Custovic; Daniel J Jackson; James E Gern; Robert F Lemanske; Stefano Guerra; Anne L Wright; Carole Ober; Marilyn Halonen; Donata Vercelli Journal: J Allergy Clin Immunol Date: 2016-12-21 Impact factor: 10.793
Authors: Christine L M Joseph; Suzanne Havstad; Kevin Bobbitt; Kimberley Woodcroft; Edward M Zoratti; Christian Nageotte; Rana Misiak; Robert Enberg; Charlotte Nicholas; Jerel M Ezell; Dennis R Ownby; Christine Cole Johnson Journal: Pediatr Allergy Immunol Date: 2014-02-13 Impact factor: 6.377