| Literature DB >> 14557062 |
Mingui Fu1, Xiaojun Zhu, Jifeng Zhang, Jian Liang, Yiming Lin, Luning Zhao, Markus U Ehrengruber, Yuqing E Chen.
Abstract
Early growth response factor 1 (Egr-1) is a key transcriptional factor to mediate gene expression after vascular injury. To better understand the role of Egr-1 in vasculature, we globally profiled Egr-1 target genes in human endothelial cells using adenoviral gene transfer and Affymetrix oligonucleotide-based microarray technology. More than 300 genes regulated by >/=3-fold with Egr-1 overexpression were identified and, partially, confirmed by Northern and Western blotting, including genes for transcriptional regulators, signaling proteins, cell cycle regulatory proteins, growth factors, and cytokines. Among them, thymus-expressed chemokine (TECK) and IP-30 were dramatically induced by Egr-1, but TNFalpha-related apoptosis inducing ligand (TRAIL) was significantly repressed by Egr-1, suggesting that Egr-1 is a key mediator of inflammation and apoptosis in vascular cells. These data provide novel Egr-1 target genes and contribute to the understanding of the role of Egr-1 in vasculature.Entities:
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Year: 2003 PMID： 14557062 DOI： 10.1016/s0378-1119(03)00730-3
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688