Literature DB >> 1454531

Oligonucleotide probes detect splicing variants in situ in Drosophila embryos.

R D Artero1, M Akam, M Pérez-Alonso.   

Abstract

We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants Ia and IIa are similar in early embryos, but differ in late embryos. Variant IVa is only detected in the CNS (ps6) at stages 16 and 17. We also present evidence indicating that the first splicing event is cotranscriptional.

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Year:  1992        PMID： 1454531      PMCID： PMC334403          DOI： 10.1093/nar/20.21.5687

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  Progression of the cell cycle through mitosis leads to abortion of nascent transcripts.

Authors:  A W Shermoen; P H O'Farrell
Journal:  Cell       Date:  1991-10-18       Impact factor: 41.582

2.  Functional dissection of Ultrabithorax proteins in D. melanogaster.

Authors:  R S Mann; D S Hogness
Journal:  Cell       Date:  1990-02-23       Impact factor: 41.582

3.  Alternative RNA splicing that is spatially regulated: generation of transcripts from the Antennapedia gene of Drosophila melanogaster with different protein-coding regions.

Authors:  V L Stroeher; J C Gaiser; R L Garber
Journal:  Mol Cell Biol       Date:  1988-10       Impact factor: 4.272

4.  Immunochemical dissection of the Ultrabithorax homeoprotein family in Drosophila melanogaster.

Authors:  A J Lopez; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-15       Impact factor: 11.205

5.  Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase.

Authors:  G G Schmitz; T Walter; R Seibl; C Kessler
Journal:  Anal Biochem       Date:  1991-01       Impact factor: 3.365

6.  The distribution of Ultrabithorax transcripts in Drosophila embryos.

Authors:  M E Akam; A Martinez-Arias
Journal:  EMBO J       Date:  1985-07       Impact factor: 11.598

7.  Molecular structure and spatial expression of a homeobox gene from the labial region of the Antennapedia-complex.

Authors:  M Mlodzik; A Fjose; W J Gehring
Journal:  EMBO J       Date:  1988-08       Impact factor: 11.598

8.  Structural complexity and evolutionary conservation of the Drosophila homeotic gene proboscipedia.

Authors:  D L Cribbs; M A Pultz; D Johnson; M Mazzulla; T C Kaufman
Journal:  EMBO J       Date:  1992-04       Impact factor: 11.598

9.  Different patterns of transcription from the two Antennapedia promoters during Drosophila embryogenesis.

Authors:  J R Bermingham; A Martinez-Arias; M G Petitt; M P Scott
Journal:  Development       Date:  1990-07       Impact factor: 6.868

10.  Alternative RNA products from the Ultrabithorax domain of the bithorax complex.

Authors:  M B O'Connor; R Binari; L A Perkins; W Bender
Journal:  EMBO J       Date:  1988-02       Impact factor: 11.598
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  10 in total

Review 1.  RNA molecules lighting up under the microscope.

Authors:  R W Dirks
Journal:  Histochem Cell Biol       Date:  1996-08       Impact factor: 4.304

2.  Molecular characterization of the zerknüllt region of the Antennapedia complex of D. subobscura.

Authors:  J Terol; M Perez-Alonso; R de Frutos
Journal:  Chromosoma       Date:  1995-05       Impact factor: 4.316

3.  Stage, tissue, and cell specific distribution of alternative Ultrabithorax mRNAs and protein isoforms in the Drosophila embryo.

Authors:  A J López; R D Artero; M Pérez-Alonso
Journal:  Rouxs Arch Dev Biol       Date:  1996-05

4.  Identification of target genes regulated by homeotic proteins in Drosophila melanogaster through genetic selection of Ultrabithorax protein-binding sites in yeast.

Authors:  G S Mastick; R McKay; T Oligino; K Donovan; A J López
Journal:  Genetics       Date:  1995-01       Impact factor: 4.562

5.  A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization.

Authors:  Ana M Blanco; Laura Rausell; Begoña Aguado; Manuel Perez-Alonso; Rubén Artero
Journal:  Nucleic Acids Res       Date:  2009-06-26       Impact factor: 16.971

6.  Evolutionary conservation of the structure and expression of alternatively spliced Ultrabithorax isoforms from Drosophila.

Authors:  H M Bomze; A J López
Journal:  Genetics       Date:  1994-03       Impact factor: 4.562

7.  Functional differences between Ultrabithorax protein isoforms in Drosophila melanogaster: evidence from elimination, substitution and ectopic expression of specific isoforms.

Authors:  V Subramaniam; H M Bomze; A J López
Journal:  Genetics       Date:  1994-03       Impact factor: 4.562

8.  Combinatorial Gene Regulatory Functions Underlie Ultraconserved Elements in Drosophila.

Authors:  Maria Warnefors; Britta Hartmann; Stefan Thomsen; Claudio R Alonso
Journal:  Mol Biol Evol       Date:  2016-05-31       Impact factor: 16.240

9.  In situ detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS.

Authors:  Magnus Stougaard; Jakob S Lohmann; Magdalena Zajac; Stephen Hamilton-Dutoit; Jørn Koch
Journal:  BMC Biotechnol       Date:  2007-10-18       Impact factor: 2.563

10.  The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system.

Authors:  Ana Rogulja-Ortmann; Joao Picao-Osorio; Casandra Villava; Pedro Patraquim; Elvira Lafuente; Julie Aspden; Stefan Thomsen; Gerhard M Technau; Claudio R Alonso
Journal:  Development       Date:  2014-05       Impact factor: 6.868
  10 in total

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