Literature DB >> 14532101

Multiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H- serotype.

Karen C Jinneman1, Ken J Yoshitomi, Stephen D Weagant.   

Abstract

A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E. coli O157:H7/H- uidA gene has been developed. There is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates. The PCR efficiencies were >/= 1.89, and as few as 6 CFU/reaction could be detected.

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Year:  2003        PMID： 14532101      PMCID： PMC201207          DOI： 10.1128/AEM.69.10.6327-6333.2003

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  34 in total

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Journal:  Mol Cell Probes       Date:  1993-04       Impact factor: 2.365

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Journal:  MMWR Morb Mortal Wkly Rep       Date:  1993-04-16       Impact factor: 17.586

7.  Optimization of a 3'-minor groove binder-DNA probe targeting the uidA gene for rapid identification of Escherichia coli O157:H7 using real-time PCR.

Authors:  Ken J Yoshitomi; Karen C Jinneman; Stephen D Weagant
Journal:  Mol Cell Probes       Date:  2003-12       Impact factor: 2.365

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Journal:  Clin Microbiol Rev       Date:  1989-01       Impact factor: 26.132

10.  A waterborne outbreak in Missouri of Escherichia coli O157:H7 associated with bloody diarrhea and death.

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  20 in total

1.  Expression of putative virulence factors of Escherichia coli O157:H7 differs in bovine and human infections.

Authors:  Rebecca A Rashid; Tami A Tabata; Melissa J Oatley; Thomas E Besser; Phillip I Tarr; Steve L Moseley
Journal:  Infect Immun       Date:  2006-07       Impact factor: 3.441

2.  Misidentification of a variant biotype of Escherichia coli O157:H7 as Escherichia fergusonii by Vitek 2 Compact.

Authors:  Leta K Crawford-Miksza; Sunee Himathongkham; Mary L Dodd; Alexandru S Badoiu; Olivia M Badoiu; Linda S Guthertz
Journal:  J Clin Microbiol       Date:  2008-12-30       Impact factor: 5.948

3.  Isolation and identification of an Enterobacter cloacae strain producing a novel subtype of Shiga toxin type 1.

Authors:  William S Probert; Cassandra McQuaid; Kimmi Schrader
Journal:  J Clin Microbiol       Date:  2014-04-23       Impact factor: 5.948

4.  Site-specific clinical evaluation of the Luminex xTAG gastrointestinal pathogen panel for detection of infectious gastroenteritis in fecal specimens.

Authors:  Anami Patel; Jose Navidad; Sanjib Bhattacharyya
Journal:  J Clin Microbiol       Date:  2014-06-04       Impact factor: 5.948

5.  Evaluation of Luminex xTAG gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance.

Authors:  Jose F Navidad; David J Griswold; M Stephen Gradus; Sanjib Bhattacharyya
Journal:  J Clin Microbiol       Date:  2013-07-12       Impact factor: 5.948

6.  Virulence inhibition by zinc in shiga-toxigenic Escherichia coli.

Authors:  John K Crane; Isaac Wyatt Byrd; Edgar C Boedeker
Journal:  Infect Immun       Date:  2011-01-18       Impact factor: 3.441

7.  Presence and characterization of shiga toxin-producing Escherichia coli and other potentially diarrheagenic E. coli strains in retail meats.

Authors:  Xiaodong Xia; Jianghong Meng; Patrick F McDermott; Sherry Ayers; Karen Blickenstaff; Thu-Thuy Tran; Jason Abbott; Jie Zheng; Shaohua Zhao
Journal:  Appl Environ Microbiol       Date:  2010-01-15       Impact factor: 4.792

8.  Endophytic Bacteria Associated with Medicinal Plant Vernonia anthelmintica: Diversity and Characterization.

Authors:  Nigora Rustamova; Atikan Wubulikasimu; Nurkhodja Mukhamedov; Yanhua Gao; Dilfuza Egamberdieva; Abulimiti Yili
Journal:  Curr Microbiol       Date:  2020-03-24       Impact factor: 2.188

9.  Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada.

Authors:  David M Goldfarb; Brent Dixon; Ioana Moldovan; Nicholas Barrowman; Kirsten Mattison; Chad Zentner; Maureen Baikie; Sabah Bidawid; Francis Chan; Robert Slinger
Journal:  Int J Circumpolar Health       Date:  2013-04-08       Impact factor: 1.228

10.  Development of a robust method for isolation of shiga toxin-positive Escherichia coli (STEC) from fecal, plant, soil and water samples from a leafy greens production region in California.

Authors:  Michael B Cooley; Michele Jay-Russell; Edward R Atwill; Diana Carychao; Kimberly Nguyen; Beatriz Quiñones; Ronak Patel; Samarpita Walker; Michelle Swimley; Edith Pierre-Jerome; Andrew G Gordus; Robert E Mandrell
Journal:  PLoS One       Date:  2013-06-06       Impact factor: 3.240
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