Literature DB >> 16790788

Expression of putative virulence factors of Escherichia coli O157:H7 differs in bovine and human infections.

Rebecca A Rashid1, Tami A Tabata, Melissa J Oatley, Thomas E Besser, Phillip I Tarr, Steve L Moseley.   

Abstract

Escherichia coli O157:H7 is a commensal organism in cattle, but it is a pathogen in humans. This differential expression of virulence suggests that specific virulence factors are regulated differently in human and bovine hosts. To test this hypothesis, relative real-time reverse transcription-PCR was used to relate the expression of several putative virulence genes (eae, espA, stx(2), rfbE, ehxA, and iha) to that of the "housekeeping" gene gnd during natural human and experimental bovine infection with E. coli O157:H7. We examined these genes in fecal samples from eight humans and four calves. iha and espA were significantly more expressed in bovine infections. rfbE and ehxA appeared to be more highly expressed in human infections, though these differences did not achieve statistical significance. Our results support the hypothesis that some virulence-associated genes of O157:H7 are differentially expressed in a host-specific manner.

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Year:  2006        PMID： 16790788      PMCID： PMC1489682          DOI： 10.1128/IAI.00299-06

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  45 in total

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2.  Quantification of enterovirus RNA in sludge samples using single tube real-time RT-PCR.

Authors:  S Monpoeho; A Dehée; B Mignotte; L Schwartzbrod; V Marechal; J C Nicolas; S Billaudel; V Férré
Journal:  Biotechniques       Date:  2000-07       Impact factor: 1.993

3.  Elastase in intestinal mucus enhances the cytotoxicity of Shiga toxin type 2d.

Authors:  J F Kokai-Kun; A R Melton-Celsa; A D O'Brien
Journal:  J Biol Chem       Date:  2000-02-04       Impact factor: 5.157

4.  Iha: a novel Escherichia coli O157:H7 adherence-conferring molecule encoded on a recently acquired chromosomal island of conserved structure.

Authors:  P I Tarr; S S Bilge; J C Vary; S Jelacic; R L Habeeb; T R Ward; M R Baylor; T E Besser
Journal:  Infect Immun       Date:  2000-03       Impact factor: 3.441

5.  Distribution of putative adhesins in different seropathotypes of Shiga toxin-producing Escherichia coli.

Authors:  Claudia Toma; Estela Martínez Espinosa; Tianyan Song; Elizabeth Miliwebsky; Isabel Chinen; Sunao Iyoda; Masaaki Iwanaga; Marta Rivas
Journal:  J Clin Microbiol       Date:  2004-11       Impact factor: 5.948

6.  The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7.

Authors:  V Burland; Y Shao; N T Perna; G Plunkett; H J Sofia; F R Blattner
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7.  Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA.

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Journal:  Nucleic Acids Res       Date:  1999-02-01       Impact factor: 16.971

8.  Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality.

Authors:  H Schmidt; C Geitz; P I Tarr; M Frosch; H Karch
Journal:  J Infect Dis       Date:  1999-01       Impact factor: 5.226

9.  Identification of host-specific colonization factors of Salmonella enterica serovar Typhimurium.

Authors:  Eirwen Morgan; June D Campbell; Sonya C Rowe; Jennie Bispham; Mark P Stevens; Alison J Bowen; Paul A Barrow; Duncan J Maskell; Timothy S Wallis
Journal:  Mol Microbiol       Date:  2004-11       Impact factor: 3.501

10.  Microarray based comparison of two Escherichia coli O157:H7 lineages.

Authors:  Scot E Dowd; Hiroshi Ishizaki
Journal:  BMC Microbiol       Date:  2006-03-15       Impact factor: 3.605
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  20 in total

1.  Differences in virulence gene expression between atypical enteropathogenic Escherichia coli strains isolated from diarrheic and healthy ruminants.

Authors:  Pilar Horcajo; Gustavo Domínguez-Bernal; Javier Carrión; Ricardo De La Fuente; José A Ruiz-Santa-Quiteria; José A Orden
Journal:  Can J Vet Res       Date:  2013-04       Impact factor: 1.310

2.  The Escherichia coli O157:H7 cattle immunoproteome includes outer membrane protein A (OmpA), a modulator of adherence to bovine rectoanal junction squamous epithelial (RSE) cells.

Authors:  Indira T Kudva; Bryan Krastins; Alfredo G Torres; Robert W Griffin; Haiqing Sheng; David A Sarracino; Carolyn J Hovde; Stephen B Calderwood; Manohar John
Journal:  Proteomics       Date:  2015-03-09       Impact factor: 3.984

3.  Shiga Toxin-Producing E. coli in Animals: Detection, Characterization, and Virulence Assessment.

Authors:  Stefanie A Barth; Rolf Bauerfeind; Christian Berens; Christian Menge
Journal:  Methods Mol Biol       Date:  2021

4.  A method for isolating and analyzing human mRNA from newborn stool.

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Review 5.  Animal models of hospital-acquired pneumonia: current practices and future perspectives.

Authors:  Kenny Bielen; Bart 's Jongers; Surbhi Malhotra-Kumar; Philippe G Jorens; Herman Goossens; Samir Kumar-Singh
Journal:  Ann Transl Med       Date:  2017-03

6.  Expression of the Escherichia coli IrgA homolog adhesin is regulated by the ferric uptake regulation protein.

Authors:  Rebecca A Rashid; Phillip I Tarr; Steve L Moseley
Journal:  Microb Pathog       Date:  2006-09-06       Impact factor: 3.738

7.  Virulence inhibition by zinc in shiga-toxigenic Escherichia coli.

Authors:  John K Crane; Isaac Wyatt Byrd; Edgar C Boedeker
Journal:  Infect Immun       Date:  2011-01-18       Impact factor: 3.441

8.  Genomic instability in regions adjacent to a highly conserved pch prophage in Escherichia coli O157:H7 generates diversity in expression patterns of the LEE pathogenicity island.

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Journal:  J Bacteriol       Date:  2009-03-27       Impact factor: 3.490

9.  Adherence of Escherichia coli O157:H7 mutants in vitro and in ligated pig intestines.

Authors:  Xianhua Yin; James R Chambers; Roger Wheatcroft; Roger P Johnson; Jing Zhu; Bianfang Liu; Carlton L Gyles
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10.  Genomic diversity of pathogenic Escherichia coli of the EHEC 2 clonal complex.

Authors:  Galeb S Abu-Ali; David W Lacher; Lukas M Wick; Weihong Qi; Thomas S Whittam
Journal:  BMC Genomics       Date:  2009-07-03       Impact factor: 3.969
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