Literature DB >> 14526032

Mutations affecting DNA-binding activity of the MexR repressor of mexR-mexA-mexB-oprM operon expression.

Kohjiro Saito1, Hiroyuki Akama, Eisaku Yoshihara, Taiji Nakae.   

Abstract

We have isolated 25 MexR mutants that retained their dimerizing ability but were unable to bind mexOP DNA. Surprisingly, 20 mutations were located in the hydrophobic core region at alpha4, W1, alpha2, alpha3, and beta2, and only 3 were in positively charged residues. These results verified that DNA binding is mediated by distinct regions of MexR and showed the importance of the hydrophobic core region of the DNA-binding domain.

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Year:  2003        PMID： 14526032      PMCID： PMC225045          DOI： 10.1128/JB.185.20.6195-6198.2003

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  17 in total

1.  Mutational analysis of MarR, the negative regulator of marRAB expression in Escherichia coli, suggests the presence of two regions required for DNA binding.

Authors:  M N Alekshun; Y S Kim; S B Levy
Journal:  Mol Microbiol       Date:  2000-03       Impact factor: 3.501

2.  The crystal structure of MarR, a regulator of multiple antibiotic resistance, at 2.3 A resolution.

Authors:  M N Alekshun; S B Levy; T R Mealy; B A Seaton; J F Head
Journal:  Nat Struct Biol       Date:  2001-08

3.  Common history at the origin of the position-function correlation in transcriptional regulators in archaea and bacteria.

Authors:  E Pérez-Rueda; J Collado-Vides
Journal:  J Mol Evol       Date:  2001-09       Impact factor: 2.395

4.  Transcriptional regulation of mexR, the repressor of Pseudomonas aeruginosa mexAB-oprM multidrug efflux pump.

Authors:  Patricia Sánchez; Fernando Rojo; José L Martínez
Journal:  FEMS Microbiol Lett       Date:  2002-01-22       Impact factor: 2.742

5.  Crystal structure of the MexR repressor of the mexRAB-oprM multidrug efflux operon of Pseudomonas aeruginosa.

Authors:  Daniel Lim; Keith Poole; Natalie C J Strynadka
Journal:  J Biol Chem       Date:  2002-05-28       Impact factor: 5.157

6.  Molecular mechanism of MexR-mediated regulation of MexAB-OprM efflux pump expression in Pseudomonas aeruginosa.

Authors:  K Saito; S Eda; H Maseda; T Nakae
Journal:  FEMS Microbiol Lett       Date:  2001-02-05       Impact factor: 2.742

7.  MexR repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa: identification of MexR binding sites in the mexA-mexR intergenic region.

Authors:  K Evans; L Adewoye; K Poole
Journal:  J Bacteriol       Date:  2001-02       Impact factor: 3.490

8.  nalB-type mutations causing the overexpression of the MexAB-OprM efflux pump are located in the mexR gene of the Pseudomonas aeruginosa chromosome.

Authors:  K Saito; H Yoneyama; T Nakae
Journal:  FEMS Microbiol Lett       Date:  1999-10-01       Impact factor: 2.742

9.  Resistance of Pseudomonas aeruginosa PAO to nalidixic acid and low levels of beta-lactam antibiotics: mapping of chromosomal genes.

Authors:  M Rella; D Haas
Journal:  Antimicrob Agents Chemother       Date:  1982-08       Impact factor: 5.191

10.  Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector.

Authors:  J P Fürste; W Pansegrau; R Frank; H Blöcker; P Scholz; M Bagdasarian; E Lanka
Journal:  Gene       Date:  1986       Impact factor: 3.688
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  17 in total

1.  Determination of target sequence bound by PapX, repressor of bacterial motility, in flhD promoter using systematic evolution of ligands by exponential enrichment (SELEX) and high throughput sequencing.

Authors:  Daniel J Reiss; Harry L T Mobley
Journal:  J Biol Chem       Date:  2011-10-28       Impact factor: 5.157

2.  The X-ray crystal structure of PA1607 from Pseudomonas aureginosa at 1.9 A resolution--a putative transcription factor.

Authors:  Edyta A L Sieminska; Xiaohui Xu; Alexei Savchenko; David A R Sanders
Journal:  Protein Sci       Date:  2007-03       Impact factor: 6.725

Review 3.  The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.

Authors:  Xian-Zhi Li; Patrick Plésiat; Hiroshi Nikaido
Journal:  Clin Microbiol Rev       Date:  2015-04       Impact factor: 26.132

4.  Interplay of efflux system, ampC, and oprD expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates.

Authors:  John Quale; Simona Bratu; Jyoti Gupta; David Landman
Journal:  Antimicrob Agents Chemother       Date:  2006-05       Impact factor: 5.191

5.  Structural study of TcaR and its complexes with multiple antibiotics from Staphylococcus epidermidis.

Authors:  Yu-Ming Chang; Wen-Yih Jeng; Tzu-Ping Ko; Yao-Jen Yeh; Cammy K-M Chen; Andrew H-J Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2010-04-26       Impact factor: 11.205

6.  Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients.

Authors:  Maria Tomás; Michel Doumith; Marina Warner; Jane F Turton; Alejandro Beceiro; German Bou; David M Livermore; Neil Woodford
Journal:  Antimicrob Agents Chemother       Date:  2010-03-01       Impact factor: 5.191

7.  Whole-Genome Sequencing Reveals Diversity of Carbapenem-Resistant Pseudomonas aeruginosa Collected through CDC's Emerging Infections Program, United States, 2016-2018.

Authors:  Richard A Stanton; Davina Campbell; Gillian A McAllister; Erin Breaker; Michelle Adamczyk; Jonathan B Daniels; Joseph D Lutgring; Maria Karlsson; Kyle Schutz; Jesse T Jacob; Lucy E Wilson; Elisabeth Vaeth; Linda Li; Ruth Lynfield; Paula M Snippes Vagnone; Erin C Phipps; Emily B Hancock; Ghinwa Dumyati; Rebecca Tsay; P Maureen Cassidy; Jacquelyn Mounsey; Julian E Grass; Sandra N Bulens; Maroya Spalding Walters; Alison Laufer Halpin
Journal:  Antimicrob Agents Chemother       Date:  2022-09-06       Impact factor: 5.938

8.  The crystal structure of MexR from Pseudomonas aeruginosa in complex with its antirepressor ArmR.

Authors:  Mark S Wilke; Markus Heller; A Louise Creagh; Charles A Haynes; Lawrence P McIntosh; Keith Poole; Natalie C J Strynadka
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-23       Impact factor: 11.205

Review 9.  Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms.

Authors:  Philip D Lister; Daniel J Wolter; Nancy D Hanson
Journal:  Clin Microbiol Rev       Date:  2009-10       Impact factor: 26.132

10.  A MexR Mutation Which Confers Aztreonam Resistance to Pseudomonas aeruginosa.

Authors:  Zhenzhen Ma; Congjuan Xu; Xinxin Zhang; Dan Wang; Xiaolei Pan; Huimin Liu; Guangbo Zhu; Fang Bai; Zhihui Cheng; Weihui Wu; Yongxin Jin
Journal:  Front Microbiol       Date:  2021-06-24       Impact factor: 5.640
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