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Literature DB >> 1452097

Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae.

L Krizková¹, J Jeloková, J Klaudíny, M Zámocký.

Abstract

A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection.

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Year: 1992 PMID： 1452097 DOI： 10.1007/bf02814562

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

21 in total

1. Cloning of Japanese quail ovalbumin cDNA in E. coli.

Authors: J Klaudiny; V Klaudinyová; J Hanes; J Simúth
Journal: Folia Biol (Praha) Date: 1989 Impact factor: 0.906

2. Regulation of protein synthesis in chick oviduct. I. Independent regulation of ovalbumin, conalbumin, ovomucoid, and lysozyme induction.

Authors: R D Palmiter
Journal: J Biol Chem Date: 1972-10-25 Impact factor: 5.157

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

4. Screening expression libraries with nonradioactive immunological probes.

Authors: S Z Wang; A Esen
Journal: Gene Date: 1985 Impact factor: 3.688

5. Complete nucleotide sequence of the chicken chromosomal ovalbumin gene and its biological significance.

Authors: S L Woo; W G Beattie; J F Catterall; A Dugaiczyk; R Staden; G G Brownlee; B W O'Malley
Journal: Biochemistry Date: 1981-10-27 Impact factor: 3.162

6. Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.

Authors: J Kyhse-Andersen
Journal: J Biochem Biophys Methods Date: 1984-12

1. Production and purification of Japanese quail ovalbumin as fusion protein with glutathione S-transferase in Escherichia coli.

Authors: J Visvaderová; S Albert; A Kosová; J Klaudiny; J Simúth
Journal: Folia Microbiol (Praha) Date: 1995 Impact factor: 2.099

1 in total

Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae.

1. Cloning of Japanese quail ovalbumin cDNA in E. coli.

2. Regulation of protein synthesis in chick oviduct. I. Independent regulation of ovalbumin, conalbumin, ovomucoid, and lysozyme induction.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

4. Screening expression libraries with nonradioactive immunological probes.

5. Complete nucleotide sequence of the chicken chromosomal ovalbumin gene and its biological significance.

6. Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.

7. An immunological method for detecting gene expression in yeast colonies.

8. Chicken ovalbumin is synthesized and secreted by Escherichia coli.

9. Synthesis of a chicken ovalbumin-like protein in the yeast Saccharomyces cerevisiae.

10. The chicken ovalbumin promoter is under negative control which is relieved by steroid hormones.

1. Production and purification of Japanese quail ovalbumin as fusion protein with glutathione S-transferase in Escherichia coli.