Literature DB >> 14519992

Construction of a homologous selectable marker gene for Lentinula edodes transformation.

Toshikazu Irie1, Toshitsugu Sato, Kumiko Saito, Yoichi Honda, Takashi Watanabe, Masaaki Kuwahara, Hitoshi Enei.   

Abstract

We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.

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Year:  2003        PMID： 14519992     DOI： 10.1271/bbb.67.2006

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  9 in total

1.  The dominant Hc.Sdh (R) carboxin-resistance gene of the ectomycorrhizal fungus Hebeloma cylindrosporum as a selectable marker for transformation.

Authors:  Chrisse Ngari; Jean-Philippe Combier; Jeanne Doré; Roland Marmeisse; Gilles Gay; Delphine Melayah
Journal:  Curr Genet       Date:  2009-02-13       Impact factor: 3.886

2.  Investigating dominant selection markers for Coprinopsis cinerea: a carboxin resistance system and re-evaluation of hygromycin and phleomycin resistance vectors.

Authors:  Sreedhar Kilaru; Catherine M Collins; Amanda J Hartley; Claire Burns; Gary D Foster; Andy M Bailey
Journal:  Curr Genet       Date:  2009-07-28       Impact factor: 3.886

3.  Enhancement of ganoderic acid accumulation by overexpression of an N-terminally truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase gene in the basidiomycete Ganoderma lucidum.

Authors:  Jun-Wei Xu; Yi-Ning Xu; Jian-Jiang Zhong
Journal:  Appl Environ Microbiol       Date:  2012-08-31       Impact factor: 4.792

4.  Transformation of an oleaginous zygomycete Mortierella alpina 1S-4 with the carboxin resistance gene conferred by mutation of the iron-sulfur subunit of succinate dehydrogenase.

Authors:  Akinori Ando; Eiji Sakuradani; Kota Horinaka; Jun Ogawa; Sakayu Shimizu
Journal:  Curr Genet       Date:  2009-05-23       Impact factor: 3.886

5.  A gene locus for targeted ectopic gene integration in Zymoseptoria tritici.

Authors:  S Kilaru; M Schuster; M Latz; S Das Gupta; N Steinberg; H Fones; S J Gurr; N J Talbot; G Steinberg
Journal:  Fungal Genet Biol       Date:  2015-06       Impact factor: 3.495

Review 6.  Current technologies and related issues for mushroom transformation.

Authors:  Sinil Kim; Byeong-Suk Ha; Hyeon-Su Ro
Journal:  Mycobiology       Date:  2015-03-31       Impact factor: 1.858

7.  Effects of Medium Composition and Genetic Background on Agrobacterium-Mediated Transformation Efficiency of Lentinula edodes.

Authors:  Lianlian Yan; Ruiping Xu; Yan Zhou; Yuhua Gong; Shenghong Dai; Haiyang Liu; Yinbing Bian
Journal:  Genes (Basel)       Date:  2019-06-19       Impact factor: 4.096

8.  Application of CRISPR/Cas9 Tools for Genome Editing in the White-Rot Fungus Dichomitus squalens.

Authors:  Joanna E Kowalczyk; Shreya Saha; Miia R Mäkelä
Journal:  Biomolecules       Date:  2021-10-15

9.  Establishment of an Efficient Polyethylene Glycol (PEG)-Mediated Transformation System in Pleurotus eryngii var. ferulae Using Comprehensive Optimization and Multiple Endogenous Promoters.

Authors:  Qi Zhang; Liting Zhao; Mengye Shen; Jingyun Liu; Youran Li; Sha Xu; Lei Chen; Guiyang Shi; Zhongyang Ding
Journal:  J Fungi (Basel)       Date:  2022-02-14
  9 in total

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