AIMS: Secreted Wnt ligands are key proteins regulating cell-cell interactions and cell growth and differentiation. These proteins, along with other components of the Wnt signalling pathway, are involved in the malignant transformation of various human cancers, including malignant melanoma. This study defines the expression of several members of the Wnt ligand family and correlates their expression with histological characteristics. METHODS: The expression of Wnt2, Wnt5a, Wnt5b, Wnt7b, and Wnt10b was defined by in situ, antisense RNA hybridisation of paraffin wax embedded sections of benign naevi and malignant melanoma. Immunoperoxidase based antibody staining was used to define the expression of frizzled (Fz) receptors. RESULTS: All naevi tested strongly expressed Wnt2, Wnt5a, Wnt7b, and Wnt10b. Melanomas characterised by small, uniform cells expressed each of the Wnts in a pattern similar to that seen for benign naevi. In contrast, melanomas characterised by large, pleomorphic cells expressed Wnt10b but did not express Wnt2 and had low levels of expression of Wnt5a. Expression of Wnt7b was variable in these melanomas. Fz receptor expression was present at a low level in normal epithelium and all naevi and melanomas. CONCLUSIONS: The expression pattern of Wnt ligands in malignant melanoma correlates with histopathological features and may provide a basis for the molecular classification of this disease.
AIMS: Secreted Wnt ligands are key proteins regulating cell-cell interactions and cell growth and differentiation. These proteins, along with other components of the Wnt signalling pathway, are involved in the malignant transformation of various humancancers, including malignant melanoma. This study defines the expression of several members of the Wnt ligand family and correlates their expression with histological characteristics. METHODS: The expression of Wnt2, Wnt5a, Wnt5b, Wnt7b, and Wnt10b was defined by in situ, antisense RNA hybridisation of paraffin wax embedded sections of benign naevi and malignant melanoma. Immunoperoxidase based antibody staining was used to define the expression of frizzled (Fz) receptors. RESULTS: All naevi tested strongly expressed Wnt2, Wnt5a, Wnt7b, and Wnt10b. Melanomas characterised by small, uniform cells expressed each of the Wnts in a pattern similar to that seen for benign naevi. In contrast, melanomas characterised by large, pleomorphic cells expressed Wnt10b but did not express Wnt2 and had low levels of expression of Wnt5a. Expression of Wnt7b was variable in these melanomas. Fz receptor expression was present at a low level in normal epithelium and all naevi and melanomas. CONCLUSIONS: The expression pattern of Wnt ligands in malignant melanoma correlates with histopathological features and may provide a basis for the molecular classification of this disease.
Authors: T Murakami; S Toda; M Fujimoto; M Ohtsuki; H R Byers; T Etoh; H Nakagawa Journal: Biochem Biophys Res Commun Date: 2001-10-19 Impact factor: 3.575
Authors: D R Welch; J E Bisi; B E Miller; D Conaway; E A Seftor; K H Yohem; L B Gilmore; R E Seftor; M Nakajima; M J Hendrix Journal: Int J Cancer Date: 1991-01-21 Impact factor: 7.396
Authors: Y Miyoshi; H Nagase; H Ando; A Horii; S Ichii; S Nakatsuru; T Aoki; Y Miki; T Mori; Y Nakamura Journal: Hum Mol Genet Date: 1992-07 Impact factor: 6.150
Authors: Anthony L Schwartz; Ramiro Malgor; Eric Dickerson; Ashani T Weeraratna; Andrzej Slominski; Jacobo Wortsman; Norikazu Harii; Aimee D Kohn; Randall T Moon; Frank L Schwartz; Douglas J Goetz; Leonard D Kohn; Kelly D McCall Journal: Clin Cancer Res Date: 2009-05-26 Impact factor: 12.531
Authors: Pei-Tzu Yang; Jamie N Anastas; Rachel A Toroni; Michi M Shinohara; Jamie M Goodson; Anja K Bosserhoff; Andy J Chien; Randall T Moon Journal: EMBO Mol Med Date: 2012-11-06 Impact factor: 12.137
Authors: Deven Etnyre; Amanda L Stone; Jason T Fong; Ryan J Jacobs; Srijayaprakash B Uppada; Gregory M Botting; Supriya Rajanna; David N Moravec; Manohar R Shambannagari; Zachary Crees; Jennifer Girard; Ceyda Bertram; Neelu Puri Journal: Cancer Biol Ther Date: 2014-06-11 Impact factor: 4.742