BACKGROUND: Although allergen-specific IgE content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering. OBJECTIVES: Analyse the degranulation capacity of immunochemically defined purified and serum IgE after challenge with anti-IgE or allergen using a rat mast cell line (RBL) transfected with the alpha-chain of the human high-affinity IgE receptor (FcepsilonRI). METHODS: Purified IgE specific for 4-hydroxy-3nitrophenylacetyl, purified IgE of unknown specificity, and sera from allergic patients sensitive to Dermatophagoides pteronyssinus and Dactylis glomerata were assessed. Degranulation was measured by a beta-hexosaminidase release assay after anti-IgE or allergen-specific challenge. RESULTS: For purified monoclonal IgE a significant correlation (r = 0.97) was found between the proportion of bound allergen-specific IgE and the strength of the degranulation response. In contrast, no correlation (r = 0.27) was detected after sensitization with serum IgE. CONCLUSION: Our studies demonstrate that mast cell activation mediated through IgE from allergic patients is a result of complex relationships that are not only dependent on allergen-specific IgE content but also relate to the capacity to efficiently sensitize and trigger the signalling responses that lead to degranulation.
BACKGROUND: Although allergen-specific IgE content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering. OBJECTIVES: Analyse the degranulation capacity of immunochemically defined purified and serum IgE after challenge with anti-IgE or allergen using a rat mast cell line (RBL) transfected with the alpha-chain of the human high-affinity IgE receptor (FcepsilonRI). METHODS: Purified IgE specific for 4-hydroxy-3nitrophenylacetyl, purified IgE of unknown specificity, and sera from allergicpatients sensitive to Dermatophagoides pteronyssinus and Dactylis glomerata were assessed. Degranulation was measured by a beta-hexosaminidase release assay after anti-IgE or allergen-specific challenge. RESULTS: For purified monoclonal IgE a significant correlation (r = 0.97) was found between the proportion of bound allergen-specific IgE and the strength of the degranulation response. In contrast, no correlation (r = 0.27) was detected after sensitization with serum IgE. CONCLUSION: Our studies demonstrate that mast cell activation mediated through IgE from allergicpatients is a result of complex relationships that are not only dependent on allergen-specific IgE content but also relate to the capacity to efficiently sensitize and trigger the signalling responses that lead to degranulation.
Authors: Anna H Nowak-Wegrzyn; Ramon Bencharitiwong; John Schwarz; Gloria David; Peyton Eggleston; Peter J Gergen; Andrew H Liu; Jacqueline A Pongracic; Sampson Sarpong; Hugh A Sampson Journal: J Allergy Clin Immunol Date: 2009-04 Impact factor: 10.793
Authors: Daniel Wan; Fernanda Ludolf; Daniel G W Alanine; Owen Stretton; Eman Ali Ali; Nafal Al-Barwary; Xiaowei Wang; Michael J Doenhoff; Adriano Mari; Colin M Fitzsimmons; David W Dunne; Ryosuke Nakamura; Guilherme C Oliveira; Marcos J C Alcocer; Franco H Falcone Journal: PLoS Negl Trop Dis Date: 2014-09-25
Authors: Karen Knipping; Peter J Simons; Laura S Buelens-Sleumer; Linda Cox; Marcel den Hartog; Niels de Jong; Reiko Teshima; Johan Garssen; Louis Boon; Léon M J Knippels Journal: PLoS One Date: 2014-08-25 Impact factor: 3.240
Authors: Mohamed Z Satti; Pierre Cahen; Per S Skov; Sarah Joseph; Frances M Jones; Colin Fitzsimmons; Karl F Hoffmann; Claus Reimert; H Curtis Kariuki; Francis Kazibwe; Joseph K Mwatha; Gachuhi Kimani; Birgitte J Vennervald; John H Ouma; Narcis B Kabatereine; David W Dunne Journal: BMC Immunol Date: 2004-04-21 Impact factor: 3.615