Literature DB >> 14508687

Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1, demonstrate enhanced resistance to powdery mildew ( Sphaerotheca pannosa).

Xiangqian Li1, Ksenjia Gasic, Bruno Cammue, Willem Broekaert, Schuyler S Korban.   

Abstract

An antimicrobial protein gene, Ace-AMP1, was introduced into Rosa hybrida cv. Carefree Beauty via Agrobacterium-mediated transformation. A total of 500 putative transgenic plants were obtained from 100 primary embryogenic calli co-cultivated with A. tumefaciens following selection on a regeneration medium containing 100 mg/l kanamycin. Polymerase chain reaction analysis of these putative transgenic lines, using primers for both Ace-AMP1 and neomycin phosphotransferase ( npt II) genes, showed that 62% of these plants were positive for both transgenes. These lines were further confirmed for stable integration of Ace-AMP1 and npt II genes by Southern blotting. Transcription of the Ace-AMP1 transgene in various transgenic rose lines was determined using Northern blotting. Transgenic rose lines inoculated with conidial spores of Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. rosae showed enhanced resistance to powdery mildew using both a detached-leaf assay and an in vivo greenhouse whole-plant assay.

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Year:  2003        PMID： 14508687     DOI： 10.1007/s00425-003-1093-5

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  13 in total

1.  The methylation patterns of chromosomal integration regions influence gene activity of transferred DNA in Petunia hybrida.

Authors:  F Pröls; P Meyer
Journal:  Plant J       Date:  1992-07       Impact factor: 6.417

2.  Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds.

Authors:  F R Terras; H M Schoofs; M F De Bolle; F Van Leuven; S B Rees; J Vanderleyden; B P Cammue; W F Broekaert
Journal:  J Biol Chem       Date:  1992-08-05       Impact factor: 5.157

3.  Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway.

Authors:  I A Penninckx; K Eggermont; F R Terras; B P Thomma; G W De Samblanx; A Buchala; J P Métraux; J M Manners; W F Broekaert
Journal:  Plant Cell       Date:  1996-12       Impact factor: 11.277

4.  Solution structure of Ace-AMP1, a potent antimicrobial protein extracted from onion seeds. Structural analogies with plant nonspecific lipid transfer proteins.

Authors:  S Tassin; W F Broekaert; D Marion; D P Acland; M Ptak; F Vovelle; P Sodano
Journal:  Biochemistry       Date:  1998-03-17       Impact factor: 3.162

Review 5.  Plant defensins.

Authors:  Bart P H J Thomma; Bruno P A Cammue; Karin Thevissen
Journal:  Planta       Date:  2002-10-08       Impact factor: 4.116

6.  MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana.

Authors:  A Chakrabarti; T R Ganapathi; P K Mukherjee; V A Bapat
Journal:  Planta       Date:  2002-12-06       Impact factor: 4.116

7.  Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco.

Authors:  G Jach; B Görnhardt; J Mundy; J Logemann; E Pinsdorf; R Leah; J Schell; C Maas
Journal:  Plant J       Date:  1995-07       Impact factor: 6.417

8.  Small cysteine-rich antifungal proteins from radish: their role in host defense.

Authors:  F R Terras; K Eggermont; V Kovaleva; N V Raikhel; R W Osborn; A Kester; S B Rees; S Torrekens; F Van Leuven; J Vanderleyden
Journal:  Plant Cell       Date:  1995-05       Impact factor: 11.277

9.  Efficient genetic transformation of red raspberry, Rubus ideaus L.

Authors:  H Mathews; W Wagoner; C Cohen; J Kellogg; R Bestwick
Journal:  Plant Cell Rep       Date:  1995-05       Impact factor: 4.570

10.  A potent antimicrobial protein from onion seeds showing sequence homology to plant lipid transfer proteins.

Authors:  B P Cammue; K Thevissen; M Hendriks; K Eggermont; I J Goderis; P Proost; J Van Damme; R W Osborn; F Guerbette; J C Kader
Journal:  Plant Physiol       Date:  1995-10       Impact factor: 8.340
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  14 in total

Review 1.  Overview and detectability of the genetic modifications in ornamental plants.

Authors:  Anne-Laure Boutigny; Nicolas Dohin; David Pornin; Mathieu Rolland
Journal:  Hortic Res       Date:  2020-02-01       Impact factor: 6.793

2.  Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes.

Authors:  Scott C Schaefer; Ksenija Gasic; Bruno Cammue; Willem Broekaert; Els J M van Damme; Willy J Peumans; Schuyler S Korban
Journal:  Planta       Date:  2005-07-27       Impact factor: 4.116

3.  Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine.

Authors:  Rongrong He; Jiao Wu; Yali Zhang; Cecilia B Agüero; Xinlong Li; Shaoli Liu; Chaoxia Wang; M Andrew Walker; Jiang Lu
Journal:  Protoplasma       Date:  2016-11-30       Impact factor: 3.356

4.  Pyramiding of nine transgenes in maize generates high-level resistance against necrotrophic maize pathogens.

Authors:  Xiang Zhu; Jinfeng Zhao; Hafiz Muhammad Khalid Abbas; Yunjun Liu; Menglan Cheng; Jue Huang; Wenjuan Cheng; Beibei Wang; Cuiying Bai; Guoying Wang; Wubei Dong
Journal:  Theor Appl Genet       Date:  2018-07-13       Impact factor: 5.699

5.  Combined expression of chitinase and lipid transfer protein genes in transgenic carrot plants enhances resistance to foliar fungal pathogens.

Authors:  J Jayaraj; Z K Punja
Journal:  Plant Cell Rep       Date:  2007-05-17       Impact factor: 4.570

6.  Deployment of Stacked Antimicrobial Genes in Banana for Stable Tolerance Against Fusarium oxysporum f.sp. cubense Through Genetic Transformation.

Authors:  C Sunisha; H D Sowmya; T R Usharani; M Umesha; H R Gopalkrishna; Arvindkumar Saxena
Journal:  Mol Biotechnol       Date:  2020-01       Impact factor: 2.695

7.  Arabidopsis thaliana plants expressing human beta-defensin-2 are more resistant to fungal attack: functional homology between plant and human defensins.

Authors:  An M Aerts; Karin Thevissen; Sara M Bresseleers; Jan Sels; Piet Wouters; Bruno P A Cammue; Isabelle E J A François
Journal:  Plant Cell Rep       Date:  2007-03-06       Impact factor: 4.570

8.  Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses.

Authors:  Subhankar Roy-Barman; Christof Sautter; Bharat B Chattoo
Journal:  Transgenic Res       Date:  2006-08       Impact factor: 3.145

Review 9.  Temporal and spatial control of gene expression in horticultural crops.

Authors:  Manjul Dutt; Sadanand A Dhekney; Leonardo Soriano; Raju Kandel; Jude W Grosser
Journal:  Hortic Res       Date:  2014-09-24       Impact factor: 6.793

10.  Identification of Powdery Mildew Responsive Genes in Hevea brasiliensis through mRNA Differential Display.

Authors:  Xiang Li; Zhenghong Bi; Rong Di; Peng Liang; Qiguang He; Wenbo Liu; Weiguo Miao; Fucong Zheng
Journal:  Int J Mol Sci       Date:  2016-01-29       Impact factor: 5.923
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