Literature DB >> 31667713

Deployment of Stacked Antimicrobial Genes in Banana for Stable Tolerance Against Fusarium oxysporum f.sp. cubense Through Genetic Transformation.

C Sunisha1, H D Sowmya2, T R Usharani3, M Umesha2, H R Gopalkrishna2, Arvindkumar Saxena2.   

Abstract

Enhanced tolerance to wilt disease (Fusarium oxysporum f.sp. cubense) was achieved in banana variety Rasthali (AAB) by the transformation of embryogenic cells with two antimicrobial genes viz., Ace-AMP1 and pflp using Agrobacterium mediated transformation. The transgene copy numbers in stable transformants were confirmed by Southern analysis. The expression of stacked genes in the transgenic lines was validated by RT-PCR as well as Northern analysis. Bioassay using Foc race 1 in pot culture experiments demonstrated enhanced tolerance after 180 days of planting. Two independent transformants showed 10-20% Vascular Discoloration Index compared to untransformed banana cv. Rasthali (96%). The stacked lines revealed higher activity of Super Oxide Dismutase and Peroxidase compared to untransformed control which depicted higher tolerance to oxidative stress caused by Foc infection.

Entities:  

Keywords:  Antimicrobial peptide; Fusarium oxysporum f.sp. cubense; Fusarium wilt; Plant ferredoxin-like protein; Rasthali

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Year:  2020        PMID： 31667713     DOI： 10.1007/s12033-019-00219-w

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  20 in total

Review 1.  Performing the paradoxical: how plant peroxidases modify the cell wall.

Authors:  Filippo Passardi; Claude Penel; Christophe Dunand
Journal:  Trends Plant Sci       Date:  2004-11       Impact factor: 18.313

2.  Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain.

Authors:  Jane Vishnevetsky; Thomas L White; Aaron J Palmateer; Moshe Flaishman; Yuval Cohen; Yigal Elad; Margarita Velcheva; Uri Hanania; Nachman Sahar; Oded Dgani; Avihai Perl
Journal:  Transgenic Res       Date:  2010-04-16       Impact factor: 2.788

3.  Functional characterization of the recombinant antimicrobial peptide Trx-Ace-AMP1 and its application on the control of tomato early blight disease.

Authors:  Yin Wu; Yue He; Xiaochun Ge
Journal:  Appl Microbiol Biotechnol       Date:  2011-03-05       Impact factor: 4.813

4.  Evidence for the Contribution of the Mehler-Peroxidase Reaction in Dissipating Excess Electrons in Drought-Stressed Wheat.

Authors:  K. Biehler; H. Fock
Journal:  Plant Physiol       Date:  1996-09       Impact factor: 8.340

5.  Ferredoxin from sweet pepper (Capsicum annuum L.) intensifying harpin(pss)-mediated hypersensitive response shows an enhanced production of active oxygen species (AOS).

Authors:  Badri Venkata Dayakar; Hao-Jan Lin; Cheng-Hsien Chen; Mang-Jye Ger; Bor-Heng Lee; Chia-Hwei Pai; David Chow; Hsiang-En Huang; Shaw-Yhi Hwang; Mei-Chu Chung; Teng-Yung Feng
Journal:  Plant Mol Biol       Date:  2003-04       Impact factor: 4.076

6.  Host-induced post-transcriptional hairpin RNA-mediated gene silencing of vital fungal genes confers efficient resistance against Fusarium wilt in banana.

Authors:  Siddhesh B Ghag; Upendra K S Shekhawat; Thumballi R Ganapathi
Journal:  Plant Biotechnol J       Date:  2014-01-30       Impact factor: 9.803

7.  Generation of active oxygen in elicited cells of Arabidopsis thaliana is mediated by a NADPH oxidase-like enzyme.

Authors:  R Desikan; J T Hancock; M J Coffey; S J Neill
Journal:  FEBS Lett       Date:  1996-03-11       Impact factor: 4.124

8.  Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses.

Authors:  Subhankar Roy-Barman; Christof Sautter; Bharat B Chattoo
Journal:  Transgenic Res       Date:  2006-08       Impact factor: 3.145

9.  Petunia floral defensins with unique prodomains as novel candidates for development of fusarium wilt resistance in transgenic banana plants.

Authors:  Siddhesh B Ghag; Upendra K Singh Shekhawat; Thumballi R Ganapathi
Journal:  PLoS One       Date:  2012-06-22       Impact factor: 3.240

10.  Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice.

Authors:  Subhasis Karmakar; Kutubuddin A Molla; Kaushik Das; Sailendra Nath Sarkar; Swapan K Datta; Karabi Datta
Journal:  Sci Rep       Date:  2017-08-11       Impact factor: 4.379
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  3 in total

1.  Analysis of TCP Transcription Factors Revealed Potential Roles in Plant Growth and Fusarium oxysporum f.sp. cubense Resistance in Banana (cv. Rasthali).

Authors:  Siddhant Chaturvedi; Shahirina Khan; T R Usharani; Siddharth Tiwari
Journal:  Appl Biochem Biotechnol       Date:  2022-07-05       Impact factor: 3.094

2.  Induction of Ced9 mediated anti-apoptosis in commercial banana cultivar Rasthali for stable resistance against Fusarium wilt.

Authors:  C Sunisha; H D Sowmya; T R Usharani; M Umesha; H R Gopalkrishna; S Sriram
Journal:  3 Biotech       Date:  2020-08-03       Impact factor: 2.406

Review 3.  Improvements in the Resistance of the Banana Species to Fusarium Wilt: A Systematic Review of Methods and Perspectives.

Authors:  Anelita de Jesus Rocha; Julianna Matos da Silva Soares; Fernanda Dos Santos Nascimento; Adriadna Souza Santos; Vanusia Batista de Oliveira Amorim; Claudia Fortes Ferreira; Fernando Haddad; Janay Almeida Dos Santos-Serejo; Edson Perito Amorim
Journal:  J Fungi (Basel)       Date:  2021-03-25
  3 in total

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