OBJECTIVE: To test the hypothesis that low concentrations of distal airspace water-soluble antioxidants are associated with acute lung injury. DESIGN: Prospective, cohort study. SETTING: Medical intensive care unit of two tertiary care hospitals. SUBJECTS: Subjects were 29 patients with acute lung injury and 23 normal, healthy, volunteers. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Pulmonary edema fluid from subjects with acute lung injury was aspirated immediately after intubation. Compared with the bronchoalveolar lavage from normal subjects (corrected for dilution using urea concentrations), undiluted edema fluid from acute lung injury subjects had significantly lower concentrations of the antioxidants urate (757 +/- 232 microM vs. 328 +/- 75 microM), glutathione (138 +/- 25 microM vs. 7 +/- 4 microM), and ascorbate (85 +/- 21 microM vs. 27 +/- 10 microM). CONCLUSIONS: Acute lung injury is associated with decreased concentrations of water-soluble antioxidants in the distal airspaces. In acute lung injury, the distal airspace antioxidants ascorbate, urate, and glutathione may play a role in attenuating lung injury.
OBJECTIVE: To test the hypothesis that low concentrations of distal airspace water-soluble antioxidants are associated with acute lung injury. DESIGN: Prospective, cohort study. SETTING: Medical intensive care unit of two tertiary care hospitals. SUBJECTS: Subjects were 29 patients with acute lung injury and 23 normal, healthy, volunteers. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS:Pulmonary edema fluid from subjects with acute lung injury was aspirated immediately after intubation. Compared with the bronchoalveolar lavage from normal subjects (corrected for dilution using urea concentrations), undiluted edema fluid from acute lung injury subjects had significantly lower concentrations of the antioxidants urate (757 +/- 232 microM vs. 328 +/- 75 microM), glutathione (138 +/- 25 microM vs. 7 +/- 4 microM), and ascorbate (85 +/- 21 microM vs. 27 +/- 10 microM). CONCLUSIONS:Acute lung injury is associated with decreased concentrations of water-soluble antioxidants in the distal airspaces. In acute lung injury, the distal airspace antioxidants ascorbate, urate, and glutathione may play a role in attenuating lung injury.
Authors: Sotirios G Zarogiannis; Asta Jurkuvenaite; Solana Fernandez; Stephen F Doran; Amit K Yadav; Giuseppe L Squadrito; Edward M Postlethwait; Larry Bowen; Sadis Matalon Journal: Am J Respir Cell Mol Biol Date: 2010-12-03 Impact factor: 6.914
Authors: Ciara M Shaver; Cameron P Upchurch; David R Janz; Brandon S Grove; Nathan D Putz; Nancy E Wickersham; Sergey I Dikalov; Lorraine B Ware; Julie A Bastarache Journal: Am J Physiol Lung Cell Mol Physiol Date: 2016-01-15 Impact factor: 5.464
Authors: Chirag Kariya; Heather Leitner; Elysia Min; Christiaan van Heeckeren; Anna van Heeckeren; Brian J Day Journal: Am J Physiol Lung Cell Mol Physiol Date: 2007-03-16 Impact factor: 5.464