Literature DB >> 14501130

Crystallization of the GMPPCP complex of the NG domains of Thermus aquaticus Ffh and FtsY.

Irina V Shepotinovskaya1, Pamela J Focia, Douglas M Freymann.   

Abstract

The GTPases Ffh and FtsY are components of the prokaryotic signal recognition particle protein-targeting pathway. The two proteins interact in a GTP-dependent manner, forming a complex that can be stabilized by use of the non-hydrolyzable GTP analog GMPPCP. Crystals of the complex of the NG GTPase domains of the two proteins have been obtained from ammonium sulfate solutions. Crystals grow with several different morphologies, predominately as poorly diffracting plates and needle clusters, but occasionally as well diffracting rods. It has been demonstrated that all forms of the crystals observed contain an intact complex. Diffraction data to 2.0 A resolution have been measured.

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Year:  2003        PMID： 14501130      PMCID： PMC3543697          DOI： 10.1107/s0907444903016573

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  22 in total

1.  A site-specific, membrane-dependent cleavage event defines the membrane binding domain of FtsY.

Authors:  J S Millman; D W Andrews
Journal:  J Biol Chem       Date:  1999-11-19       Impact factor: 5.157

2.  Structure of the conserved GTPase domain of the signal recognition particle.

Authors:  D M Freymann; R J Keenan; R M Stroud; P Walter
Journal:  Nature       Date:  1997-01-23       Impact factor: 49.962

3.  Ffh and FtsY in a Mycoplasma mycoides signal-recognition particle pathway: SRP RNA and M domain of Ffh are not required for stimulation of GTPase activity in vitro.

Authors:  B Macao; J Luirink; T Samuelsson
Journal:  Mol Microbiol       Date:  1997-05       Impact factor: 3.501

4.  The NG domain of the prokaryotic signal recognition particle receptor, FtsY, is fully functional when fused to an unrelated integral membrane polypeptide.

Authors:  A Zelazny; A Seluanov; A Cooper; E Bibi
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-10       Impact factor: 11.205

5.  Membrane association of FtsY, the E. coli SRP receptor.

Authors:  E de Leeuw; D Poland; O Mol; I Sinning; C M ten Hagen-Jongman; B Oudega; J Luirink
Journal:  FEBS Lett       Date:  1997-10-27       Impact factor: 4.124

6.  Conformational change of the N-domain on formation of the complex between the GTPase domains of Thermus aquaticus Ffh and FtsY.

Authors:  Irina V Shepotinovskaya; Douglas M Freymann
Journal:  Biochim Biophys Acta       Date:  2002-05-20

7.  Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle.

Authors:  H D Bernstein; M A Poritz; K Strub; P J Hoben; S Brenner; P Walter
Journal:  Nature       Date:  1989-08-10       Impact factor: 49.962

8.  Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor.

Authors:  J D Miller; H D Bernstein; P Walter
Journal:  Nature       Date:  1994-02-17       Impact factor: 49.962

9.  Reciprocal stimulation of GTP hydrolysis by two directly interacting GTPases.

Authors:  T Powers; P Walter
Journal:  Science       Date:  1995-09-08       Impact factor: 47.728

10.  GTPase domain of the 54-kD subunit of the mammalian signal recognition particle is required for protein translocation but not for signal sequence binding.

Authors:  D Zopf; H D Bernstein; P Walter
Journal:  J Cell Biol       Date:  1993-03       Impact factor: 10.539
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  8 in total

1.  Heterodimeric GTPase core of the SRP targeting complex.

Authors:  Pamela J Focia; Irina V Shepotinovskaya; James A Seidler; Douglas M Freymann
Journal:  Science       Date:  2004-01-16       Impact factor: 47.728

2.  Structure of a GDP:AlF4 complex of the SRP GTPases Ffh and FtsY, and identification of a peripheral nucleotide interaction site.

Authors:  Pamela J Focia; Joseph Gawronski-Salerno; John S Coon; Douglas M Freymann
Journal:  J Mol Biol       Date:  2006-05-26       Impact factor: 5.469

3.  X-ray structure of the T. aquaticus FtsY:GDP complex suggests functional roles for the C-terminal helix of the SRP GTPases.

Authors:  Joseph Gawronski-Salerno; John S Coon; Pamela J Focia; Douglas M Freymann
Journal:  Proteins       Date:  2007-03-01

4.  Structure of the GMPPNP-stabilized NG domain complex of the SRP GTPases Ffh and FtsY.

Authors:  Joseph Gawronski-Salerno; Douglas M Freymann
Journal:  J Struct Biol       Date:  2006-11-03       Impact factor: 2.867

5.  SRP RNA provides the physiologically essential GTPase activation function in cotranslational protein targeting.

Authors:  Fai Y Siu; Richard J Spanggord; Jennifer A Doudna
Journal:  RNA       Date:  2006-12-12       Impact factor: 4.942

6.  Analysis of protein hydration in ultrahigh-resolution structures of the SRP GTPase Ffh.

Authors:  Ursula D Ramirez; Douglas M Freymann
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2006-11-23

7.  Two-step membrane binding by the bacterial SRP receptor enable efficient and accurate Co-translational protein targeting.

Authors:  Yu-Hsien Hwang Fu; William Y C Huang; Kuang Shen; Jay T Groves; Thomas Miller; Shu-Ou Shan
Journal:  Elife       Date:  2017-07-28       Impact factor: 8.140

8.  Nucleotide-binding flexibility in ultrahigh-resolution structures of the SRP GTPase Ffh.

Authors:  Ursula D Ramirez; Pamela J Focia; Douglas M Freymann
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2008-09-19
  8 in total

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