| Literature DB >> 14500843 |
Andrew J Flavell1, Viacheslav N Bolshakov, Allan Booth, Runchun Jing, Joanne Russell, T H Noel Ellis, Peter Isaac.
Abstract
A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotin- terminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides to tags attached to the allele- specific PCR primers. Two tagged primer oligonucleotides are used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with multiple fluorochromes.Entities:
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Year: 2003 PMID： 14500843 PMCID： PMC206481 DOI： 10.1093/nar/gng113
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971