Literature DB >> 1445217

Purification and characterization of a diptericin homologue from Sarcophaga peregrina (flesh fly).

M Ishikawa1, T Kubo, S Natori.   

Abstract

A protein with a molecular mass of 8 kDa was found to be synthesized specifically when the fat-body from injured Sarcophaga peregrina larvae was cultured in vitro. This protein was purified from the haemolymph of the injured larvae to near-homogeneity. Partial amino acid sequencing revealed that this protein is a diptericin homologue. It showed bactericidal activity on growing, but not resting Escherichia coli cells. E. coli cells become elongated on treatment with this protein.

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Year:  1992        PMID： 1445217      PMCID： PMC1133203          DOI： 10.1042/bj2870573

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  24 in total

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Authors:  S W Kessler
Journal:  J Immunol       Date:  1975-12       Impact factor: 5.422

Review 2.  Cell-free immunity in Cecropia. A model system for antibacterial proteins.

Authors:  H G Boman; I Faye; G H Gudmundsson; J Y Lee; D A Lidholm
Journal:  Eur J Biochem       Date:  1991-10-01

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Purification of sarcotoxin II, antibacterial proteins of Sarcophaga peregrina (flesh fly) larvae.

Authors:  K Ando; M Okada; S Natori
Journal:  Biochemistry       Date:  1987-01-13       Impact factor: 3.162

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
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Review 6.  Cell-free immunity in insects.

Authors:  H G Boman; D Hultmark
Journal:  Annu Rev Microbiol       Date:  1987       Impact factor: 15.500

7.  Primary structure of sarcotoxin I, an antibacterial protein induced in the hemolymph of Sarcophaga peregrina (flesh fly) larvae.

Authors:  M Okada; S Natori
Journal:  J Biol Chem       Date:  1985-06-25       Impact factor: 5.157

8.  Insect immunity. Characterization of a Drosophila cDNA encoding a novel member of the diptericin family of immune peptides.

Authors:  C Wicker; J M Reichhart; D Hoffmann; D Hultmark; C Samakovlis; J A Hoffmann
Journal:  J Biol Chem       Date:  1990-12-25       Impact factor: 5.157

9.  Measurement of Sarcophaga peregrina lectin under various physiological conditions by radioimmunoassay.

Authors:  H Komano; R Nozawa; D Mizuno; S Natori
Journal:  J Biol Chem       Date:  1983-02-25       Impact factor: 5.157

10.  Insect immunity. Purification and characterization of a family of novel inducible antibacterial proteins from immunized larvae of the dipteran Phormia terranovae and complete amino-acid sequence of the predominant member, diptericin A.

Authors:  J L Dimarcq; E Keppi; B Dunbar; J Lambert; J M Reichhart; D Hoffmann; S M Rankine; J E Fothergill; J A Hoffmann
Journal:  Eur J Biochem       Date:  1988-01-15
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  7 in total

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