Literature DB >> 1438143

Phosphorylation of Toxoplasma gondii major surface antigens.

S Tomavo1, A Martinage, J F Dubremetz.   

Abstract

The phosphorylation of the major surface proteins of Toxoplasma gondii tachyzoites was investigated. Metabolic labeling of intracellular tachyzoites with [32P]-orthophosphate followed by immunoprecipitation with specific monoclonal antibodies showed that all five major surface proteins were labeled as expected for GPI-anchored proteins. More detailed analysis of the phosphorylated sites revealed that surface proteins P30 and P22 contained phosphoserine residues in addition to the phosphorylated molecules that are presumably localized in the GPI-membrane anchors.

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Year:  1992        PMID： 1438143     DOI： 10.1007/bf00936449

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  12 in total

Review 1.  Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures.

Authors:  M A Ferguson; A F Williams
Journal:  Annu Rev Biochem       Date:  1988       Impact factor: 23.643

Review 2.  Identification and analysis of glycoinositol phospholipid anchors in membrane proteins.

Authors:  T L Rosenberry; J P Toutant; R Haas; W L Roberts
Journal:  Methods Cell Biol       Date:  1989       Impact factor: 1.441

3.  Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii.

Authors:  J L Burg; D Perelman; L H Kasper; P L Ware; J C Boothroyd
Journal:  J Immunol       Date:  1988-11-15       Impact factor: 5.422

Review 4.  Biochemistry of the glycosyl-phosphatidylinositol membrane protein anchors.

Authors:  M G Low
Journal:  Biochem J       Date:  1987-05-15       Impact factor: 3.857

5.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Purification of a major membrane protein of Toxoplasma gondii by immunoabsorption with a monoclonal antibody.

Authors:  L H Kasper; J H Crabb; E R Pfefferkorn
Journal:  J Immunol       Date:  1983-05       Impact factor: 5.422

8.  Isolation and characterization of a monoclonal antibody-resistant antigenic mutant of Toxoplasma gondii.

Authors:  L H Kasper; J H Crabb; E R Pfefferkorn
Journal:  J Immunol       Date:  1982-10       Impact factor: 5.422

9.  Detection and characterization of membrane antigens of Toxoplasma gondii.

Authors:  E Handman; J W Goding; J S Remington
Journal:  J Immunol       Date:  1980-06       Impact factor: 5.422

10.  The major surface antigen, P30, of Toxoplasma gondii is anchored by a glycolipid.

Authors:  S D Nagel; J C Boothroyd
Journal:  J Biol Chem       Date:  1989-04-05       Impact factor: 5.157
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  2 in total

1.  The Toxoplasma gondii rhoptry protein ROP4 is secreted into the parasitophorous vacuole and becomes phosphorylated in infected cells.

Authors:  Kimberly L Carey; Artemio M Jongco; Kami Kim; Gary E Ward
Journal:  Eukaryot Cell       Date:  2004-10

2.  Recombinant Toxoplasma gondii surface antigen 1 (P30) expressed in Escherichia coli is recognized by human Toxoplasma-specific immunoglobulin M (IgM) and IgG antibodies.

Authors:  D Harning; J Spenter; A Metsis; J Vuust; E Petersen
Journal:  Clin Diagn Lab Immunol       Date:  1996-05
  2 in total

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