| Literature DB >> 1435729 |
V M Lenzini1, J Magdalena, C Fraipont, B Joris, A Matagne, J Dusart.
Abstract
The previously cloned class A beta-lactamase gene (bla) of Streptomyces cacaoi was shown to be inducible by beta-lactam compounds in the host organism S. lividans. A regulatory region of 2.75 kb was identified and the nucleotide sequence determined. It contained four open reading frames (ORFs) of which only two were complete and required for induction. ORF1-ORF2 exerted a positive regulatory effect on the expression of bla. Inactivation of ORF1 or of ORF2 resulted not only in the loss of induction, but also in a 30- to 60-fold decrease in the basal (non-induced) level of beta-lactamase production. ORF1 codes for a DNA-binding protein related to the AmpR repressor/activator, which controls the expression of ampC (class C beta-lactamase) genes in several Enterobacteria.Entities:
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Year: 1992 PMID： 1435729 DOI： 10.1007/bf00286179
Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925