Literature DB >> 14342494

THE ESTIMATION OF DEOXYRIBONUCLEIC ACID IN THE PRESENCE OF SIALIC ACID: APPLICATION TO ANALYSIS OF HUMAN GASTRIC WASHINGS.

D N CROFT, M LUBRAN.   

Abstract

1. Sialic acid has been found to interfere with three colorimetric reactions used for the estimation of DNA: a modified diphenylamine reaction at 100 degrees (Dische, 1930), the nitrophenylhydrazine method (Webb & Levy, 1955) and the diphenylamine reaction at 30 degrees (Burton, 1956). 2. Evidence is presented that sialic acid is present in hydrolysates obtained from gastric wash-out material. 3. A mathematical method for correcting for interference from sialic acid in the diphenylamine reaction at 30 degrees is described. 4. The diphenylamine reaction has been modified to make it suitable for the estimation of DNA in the presence of sialic acid. The modifications are to increase the concentration of diphenylamine to 2% and to perform the reaction at 6-13 degrees for 48hr. These modifications increase the sensitivity 25% above Burton's (1956) modification of the diphenylamine reaction. 5. The precipitation, extraction and recovery of DNA from gastric wash-out material have been investigated.

Entities:  

Keywords:  ANILINE COMPOUNDS; BIOCHEMISTRY; COLORIMETRY; DNA; GASTRIC LAVAGE; INDICATORS AND REAGENTS; NEURAMINIC ACIDS; STOMACH

Mesh：

Substances：

Year:  1965        PMID： 14342494      PMCID： PMC1206785          DOI： 10.1042/bj0950612

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  5 in total

1.  COMPOUNDS INTERFERING IN THE DIPHENYLAMINE TEST FOR DEOXYRIBONUCLEIC ACID.

Authors:  J B LEE
Journal:  Nature       Date:  1963-10-19       Impact factor: 49.962

2.  ASPIRIN AND THE EXFOLIATION OF GASTRIC EPITHELIAL CELLS. CYTOLOGICAL AND BIOCHEMICAL OBSERVATIONS.

Authors:  D N CROFT
Journal:  Br Med J       Date:  1963-10-12

3.  A sensitive method for the determination of deoxyribonucleic acid in tissues and microorganisms.

Authors:  J M WEBB; H B LEVY
Journal:  J Biol Chem       Date:  1955-03       Impact factor: 5.157

4.  New developments in the chemical determination of nucleic acids.

Authors:  J M WEBB; H B LEVY
Journal:  Methods Biochem Anal       Date:  1958

5.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857
  5 in total
  72 in total

1.  Cell turnover and loss and the gastric mucosal barrier.

Authors:  D N Croft
Journal:  Am J Dig Dis       Date:  1977-04

2.  Effect of intragastric infusion of tobacco powder on DNA content of gastric aspirate.

Authors:  H G Desai; K Venugopalan; F P Anita
Journal:  Am J Dig Dis       Date:  1975-05

3.  Effects of erdosteine on sputum biochemical and rheologic properties: pharmacokinetics in chronic obstructive lung disease.

Authors:  C F Marchioni; M Moretti; M Muratori; M C Casadei; P Guerzoni; R Scuri; G B Fregnan
Journal:  Lung       Date:  1990       Impact factor: 2.584

4.  Enzymatic inactivation of bradykinin by rat brain neuronal perikarya.

Authors:  E A DelBel; A P Padovan; G J Padovan; O Z Sellinger; A R Martins
Journal:  Cell Mol Neurobiol       Date:  1989-09       Impact factor: 5.046

5.  Fractionation of nuclei from brain by zonal centrifugation and a study of the ribonucleic acid polymerase activity in the various classes of nuclei.

Authors:  J Austoker; D Cox; A P Mathias
Journal:  Biochem J       Date:  1972-10       Impact factor: 3.857

6.  An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.

Authors:  M R Adelman; G Blobel; D D Sabatini
Journal:  J Cell Biol       Date:  1973-01       Impact factor: 10.539

7.  The activity of deoxyribonucleic acid polymerase and deoxyribonucleic acid synthesis in nuclei from brain fractionated by zonal centrifugation.

Authors:  M A Stambolova; D Cox; A P Mathias
Journal:  Biochem J       Date:  1973-11       Impact factor: 3.857

8.  Measurement of glycoprotein content and cell kinetics in preulcerous gastric mucosa.

Authors:  F Andre; C Andre; S Fournier
Journal:  Dig Dis Sci       Date:  1979-09       Impact factor: 3.199

9.  The occurrence of two types of deoxyribonucleic acid polymerase activity in the main classes of nuclei obtained from the brains of infant and adult rats.

Authors:  M A Stambolova; D Cox; A P Mathias
Journal:  Biochem J       Date:  1974-04       Impact factor: 3.857

10.  Small-bowel resection. Oral intake is the stimulus for hyperplasia.

Authors:  G M Levine; J J Deren; E Yezdimir
Journal:  Am J Dig Dis       Date:  1976-07
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