| Literature DB >> 14342236 |
Abstract
1. The presence of palmitoyl-CoA-l-glycerol 1-phosphate palmitoyltransferase (EC2.3.1.15) has been demonstrated in a particulate fraction of baker's yeast. 2. The enzyme has been characterized, and its activity studied as a function of pH and concentration of substrates. 3. Inhibition by thiol poisons and protection by acyl-CoA have been used to obtain information on the active site. 4. By various methods of supplying acyl radicals, the species ;palmitoyl-CoA' has been shown to be the true acyl donor to the transferase.Entities:
Keywords: ACYLTRANSFERASES; AMIDES; CATALYSIS; CHROMATOGRAPHY; COENZYME A; ENZYME INHIBITORS; ETHYLMALEIMIDE; EXPERIMENTAL LAB STUDY; GLYCEROPHOSPHATES; HYDROGEN-ION CONCENTRATION; LIGASES; OLEIC ACID; PALMITIC ACID; PHARMACOLOGY; PHOSPHOLIPID METABOLISM; PYRROLES; SACCHAROMYCES
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Year: 1965 PMID: 14342236 PMCID: PMC1206433 DOI: 10.1042/bj0940240
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857