| Literature DB >> 1433257 |
A R Holmes1, Y C Lee, R D Cannon, H F Jenkinson, M G Shepherd.
Abstract
Two DNA fragments cloned from the genome of Candida albicans ATCC 10261 may be useful in the rapid diagnosis of disseminated candidosis. One sequence (probe EOB1) was specific for C. albicans (positive hybridisation with 45 strains tested). The second sequence (probe EOB2) detected C. albicans, as well as five other pathogenic Candida spp. and Saccharomyces cerevisiae, but did not react with human or bacterial DNA. Both probes were repetitive sequences in the genome of C. albicans. Probe EOB1 was used to detect, without DNA amplification, 500 C. albicans yeast cells in 1 ml of human blood.Entities:
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Year: 1992 PMID： 1433257 DOI： 10.1099/00222615-37-5-346
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472