INFLUENCE OF DEOXYRIBONUCLEIC ACID DEGRADATION PRODUCTS AND ORTHOPHOSPHATE ON UPTAKE OF DEOXYNUCLEOSIDES IN PNEUMOCOCCUS TYPE 3.

Firshein, William (Wesleyan University, Middletown, Conn.), and Bernadette A. Gargan. Influence of deoxyribonucleic acid degradation products and orthophosphate on uptake of deoxynucleosides in pneumococcus type III. J. Bacteriol. 90:337-342. 1965.-The uptake of specific C(14)-deoxynucleosides by a type III encapsulated (virulent) strain of pneumococcus in resting-cell suspensions is enhanced when such deoxynucleosides are added as part of a supplement consisting of other deoxynucleosides, deoxynucleotides, an oligo(deoxy)nucleotide fraction derived from a deoxyribonuclease-treated calf-thymus deoxyribonucleic acid, and orthophosphate. Ribonucleic acid degradation products or yeast extract are ineffective in this respect. Mixtures of deoxynucleosides and deoxynucleotides stimulate uptake of a specific deoxynucleoside during early stages of incubation, whereas orthophosphate and, to a limited extent, the oligonucleotides stimulate uptake during later stages of incubation. The stimulated uptake of specific deoxynucleosides can be abolished by adding chloramphenicol.