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Literature DB >> 14327

Fluoresceinylthiocarbamyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physicochemical studies.

Abstract

Fluoresceinylthiocarbamyl-tRNATyr (FTC-tRNATyr) is prepared from tRNATyr and fluoresceinisothiogyanate (FITC) under mildly alkaline conditions. Labelling occurs specificly at the base Q of tRNATyr. The modified tRNA is fully active in the aminoacylation assay; when aminoacylated it is recognized by the elongation factor Tu (EF-Tu). Codon-anticodon interaction, however, is severely affected by the modification.

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Year: 1977 PMID： 14327 PMCID： PMC342435 DOI： 10.1093/nar/4.2.327

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

10 in total

1. Distinct steps in the specific binding of tRNA to aminoacyl-tRNA synthetase. Temperature-jump studies on the serine-specific system from yeast and the tyrosine-specific system from Escherichia coli.

Authors: D Riesner; A Pingoud; D Boehme; F Peters; G Maass
Journal: Eur J Biochem Date: 1976-09

2. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's.

Authors: H Kasai; Y Kuchino; K Nihei; S Nishimura
Journal: Nucleic Acids Res Date: 1975-10 Impact factor: 16.971

3. Stability studies on fluorescein isothiocyanate--bovine serum albumin conjugate.

Authors: A M Landel
Journal: Anal Biochem Date: 1976-06 Impact factor: 3.365

4. Studies on the fluorescence of the Y base of yeast phenylalanine transfer ribonucleic acid. Effect of pH, aminoacylation, and interaction with elongation factor Tu.

Authors: L Beres; J Lucas-Lenard
Journal: Biochemistry Date: 1973-09-25 Impact factor: 3.162

5. The anticodon-anticodon complex.

Authors: J Eisinger; N Gross
Journal: J Mol Biol Date: 1974-09-05 Impact factor: 5.469

6. Partial degradation of transfer RNAs and transfer RNA fragments by spleen phosphodiesterase as studied by disc electrophoretic methods.

Authors: P Philippsen; G Zachau
Journal: Biochim Biophys Acta Date: 1972-09-14

7. Base analysis of ribopolynucleotides by chemical tritium labeling: a methodological study with model nucleosides and purified tRNA species.

Authors: E Randerath; C T Yu; K Randerath
Journal: Anal Biochem Date: 1972-07 Impact factor: 3.365

8. Studies on the purification and properties of factor Tu from E. coli.

Authors: D L Miller; H Weissbach
Journal: Arch Biochem Biophys Date: 1970-11 Impact factor: 4.013

9. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine.

Authors: H Kasai; Z Oashi; F Harada; S Nishimura; N J Oppenheimer; P F Crain; J G Liehr; D L von Minden; J A McCloskey
Journal: Biochemistry Date: 1975-09-23 Impact factor: 3.162

10. Nucleotide sequence of valine tRNA 1 from Escherichia coli B.

Authors: F Harada; F Kimura; S Nishimura
Journal: Biochim Biophys Acta Date: 1969-12-16

10 in total

6 in total

1. Specific fluorescent labeling of 7-(aminomethyl)-7-deazaguanosine located in the anticodon of tRNATyr isolated from E. coli mutant.

Authors: H Kasai; N Shindo-Okada; S Noguchi; S Nishimura
Journal: Nucleic Acids Res Date: 1979-09-11 Impact factor: 16.971