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Literature DB >> 14292994

FATE OF TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS.

G VENEMA, R H PRITCHARD, T VENEMA-SCHROEDER.

Abstract

Venema, G. (Hammersmith Hospital, London, England), R. H. Prtichard, and T. Venema-Schröder. Fate of transforming deoxyribonucleic acid in Bacillus subtilis. J. Bacteriol. 89:1250-1255. 1965.-Donor deoxyribonucleic acid (DNA) introduced into competent recipient cells of Bacillus subtilis undergoes a transient loss of its transforming activity shortly after uptake; transforming activity reappears as a function of time of incubation. Soon after introduction of donor DNA into the cells, joint activity of donor and recipient markers is present; this activity also increases as a function of time of incubation. However, donor marker recovery and appearance of recombinant-type activity have different kinetics, suggesting that recovery of activity of donor DNA after the eclipse phase and integration are separate processes, and that recovery precedes integration. The appearance of recombinant-type activity is independent of DNA synthesis. It is suggested that only one strand of the donor DNA is integrated in transformation. It is, therefore, possible that the eclipse is due to a phase in the transformation process during which the donor DNA is single stranded.

Entities: Species

Keywords: BACILLUS SUBTILIS; DEOXYRIBONUCLEASE; DNA, BACTERIAL; EXPERIMENTAL LAB STUDY; GENETICS; HEAT; KINETICS; METABOLISM; SPECTROPHOTOMETRY

Mesh：

Substances：

Year: 1965 PMID： 14292994 PMCID： PMC277636 DOI： 10.1128/jb.89.5.1250-1255.1965

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

1. Genetic transformation. II. The significance of damage to the DNA molecule.

Authors: L S LERMAN; L J TOLMACH
Journal: Biochim Biophys Acta Date: 1959-06

2. ON THE ROLE OF INTEGRITY OF DNA PARTICLES IN GENETIC RECOMBINATION DURING PNEUMOCOCCAL TRANSFORMATION.

Authors: J L KENT; M ROGER; R D HOTCHKISS
Journal: Proc Natl Acad Sci U S A Date: 1963-10 Impact factor: 11.205

3. The fate of transforming deoxyribonucleate following fixation by transformable bacteria. III.

Authors: M S FOX
Journal: Proc Natl Acad Sci U S A Date: 1962-06-15 Impact factor: 11.205

4. Recombination during transformation in Hemophilus influenzae.

Authors: M J VOLL; S H GOODGAL
Journal: Proc Natl Acad Sci U S A Date: 1961-04-15 Impact factor: 11.205

5. Fate of transforming deoxyribonucleate following fixation by transformable bacteria.

Authors: M S FOX; R D HOTCHKISS
Journal: Nature Date: 1960-09-17 Impact factor: 49.962

6. A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein.

Authors: K S KIRBY
Journal: Biochem J Date: 1957-07 Impact factor: 3.857

7. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors: K BURTON
Journal: Biochem J Date: 1956-02 Impact factor: 3.857

8. FORMATION OF HETEROZYGOTES BY ANNEALING A MIXTURE OF TRANSFORMING DNAS.

Authors: R M Herriott
Journal: Proc Natl Acad Sci U S A Date: 1961-02 Impact factor: 11.205

9. STRAND SEPARATION AND SPECIFIC RECOMBINATION IN DEOXYRIBONUCLEIC ACIDS: BIOLOGICAL STUDIES.

Authors: J Marmur; D Lane
Journal: Proc Natl Acad Sci U S A Date: 1960-04 Impact factor: 11.205

10. Molecular fate of DNA in genetic transformation of Pneumococcus.

Authors: S LACKS
Journal: J Mol Biol Date: 1962-07 Impact factor: 5.469

60 in total

1. The pleiotropic response regulator DegU functions as a priming protein in competence development in Bacillus subtilis.

Authors: L W Hamoen; A F Van Werkhoven; G Venema; D Dubnau
Journal: Proc Natl Acad Sci U S A Date: 2000-08-01 Impact factor: 11.205

2. Single-strand regions in the deoxyribonucleic acid of competent Haemophilus influenzae.

Authors: J E LeClerc; J K Setlow
Journal: J Bacteriol Date: 1975-06 Impact factor: 3.490

3. Lethal effect of protamine and histone on competent Bacillus subtilis cells. Inhibition of genetic transformation by protamine in sublethal concentration.

Authors: S Antohi; A Popescu
Journal: Mol Gen Genet Date: 1979-03-05

4. Improving the predictive value of the competence transcription factor (ComK) binding site in Bacillus subtilis using a genomic approach.

Authors: Leendert W Hamoen; Wiep Klaas Smits; Anne de Jong; Siger Holsappel; Oscar P Kuipers
Journal: Nucleic Acids Res Date: 2002-12-15 Impact factor: 16.971

5. The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis Marburg.

Authors: P Haima; S Bron; G Venema
Journal: Mol Gen Genet Date: 1987-09

6. Competent Bacillus subtilis cultures synthesize a denatured DNA binding activity.

Authors: E Eisenstadt; R Lange; K Willecke
Journal: Proc Natl Acad Sci U S A Date: 1975-01 Impact factor: 11.205

7. Integration of vector-containing Bacillus subtilis chromosomal DNA by a Campbell-like mechanism.

Authors: B Vosman; J Kooistra; J Olijve; G Venema
Journal: Mol Gen Genet Date: 1986-09

8. A single, specific thymine mutation in the ComK-binding site severely decreases binding and transcription activation by the competence transcription factor ComK of Bacillus subtilis.

Authors: Kim A Susanna; Aleksandra M Mironczuk; Wiep Klaas Smits; Leendert W Hamoen; Oscar P Kuipers
Journal: J Bacteriol Date: 2007-04-27 Impact factor: 3.490

9. On the effect of glucono-delta-lactone on the yeast Pichia polymorpha.

Authors: T G Villa; V Notario; T Benítez; J R Villanueva
Journal: Arch Microbiol Date: 1976-08 Impact factor: 2.552

10. PROPERTIES OF NEWLY INTRODUCED TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS.

Authors: G VENEMA; R H PRITCHARD; T VENEMA-SCHROEDER
Journal: J Bacteriol Date: 1965-08 Impact factor: 3.490