Literature DB >> 14268956

CHROMATOGRAPHIC SEPARATION OF ANNEALED AND ENZYMATICALLY SYNTHESIZED RNA-DNA HYBRIDS.

M N HAYASHI, M HAYASHI, S SPIEGELMAN.   

Abstract

A procedure is described for the chromatographic detection and isolation of DNA-RNA hybrids on columns of methylated albumin coated on kieselguhr (MAK). Its use is illustrated with both annealed and enzymatically synthesized hybrids. The method has the advantage of a wide range in capacity and resolution and permits actual isolation of the hybrid structure. It is uniquely effective in experiments involving hybridization with small DNA fragments.

Keywords:  CHROMATOGRAPHY; COLIPHAGES; DNA, BACTERIAL; DNA, VIRAL; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; HEAT; HYBRIDIZATION; METABOLISM; PHOSPHORUS ISOTOPES; RIBONUCLEASE; RNA, BACTERIAL; RNA, VIRAL; TRITIUM; ULTRACENTRIFUGATION; URACIL NUCLEOTIDES

Mesh：

Substances：

Year:  1965        PMID： 14268956      PMCID： PMC1367720          DOI： 10.1016/s0006-3495(65)86713-3

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  26 in total

1.  Fractionation of nucleic acids with the methylated albumin column.

Authors:  N SUEOKA; T Y CHENG
Journal:  J Mol Biol       Date:  1962-03       Impact factor: 5.469

2.  A method for the detection of RNA-DNA complexes.

Authors:  A P NYGAARD; B D HALL
Journal:  Biochem Biophys Res Commun       Date:  1963-07-18       Impact factor: 3.575

3.  The isolation of T4-specific RNA on a DNA-cellulose column.

Authors:  E K BAUTZ; B D HALL
Journal:  Proc Natl Acad Sci U S A       Date:  1962-03-15       Impact factor: 11.205

4.  A general method for the isolation of RNA complementary to DNA.

Authors:  E T BOLTON; B J McCARTHY
Journal:  Proc Natl Acad Sci U S A       Date:  1962-08       Impact factor: 11.205

5.  Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli.

Authors:  M CHAMBERLIN; P BERG
Journal:  Proc Natl Acad Sci U S A       Date:  1962-01-15       Impact factor: 11.205

6.  Molecular and biological characterization of messenger RNA.

Authors:  F GROS; W GILBERT; H H HIATT; G ATTARDI; P F SPAHR; J D WATSON
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1961

7.  The selective synthesis of informational RNA in bacteria.

Authors:  M HAYASHI; S SPIEGELMAN
Journal:  Proc Natl Acad Sci U S A       Date:  1961-10-15       Impact factor: 11.205

8.  Sequence complementarity of T2-DNA and T2-specific RNA.

Authors:  B D HALL; S SPIEGELMAN
Journal:  Proc Natl Acad Sci U S A       Date:  1961-02-15       Impact factor: 11.205

9.  The reaction of formaldehyde with nucleotides and T2 bacteriophage DNA.

Authors:  L GROSSMAN; S S LEVINE; W S ALLISON
Journal:  J Mol Biol       Date:  1961-02       Impact factor: 5.469

10.  The enzymatic synthesis of RNA: complementary interaction with DNA.

Authors:  E P GEIDUSCHEK; T NAKAMOTO; S B WEISS
Journal:  Proc Natl Acad Sci U S A       Date:  1961-09-15       Impact factor: 11.205
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  14 in total

1.  Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease.

Authors:  J N Hansen; B H Pheiffer; C J Hough
Journal:  Nucleic Acids Res       Date:  1974-06       Impact factor: 16.971

2.  A chemical and physical characterization of echinoid RNA during early embryogenesis.

Authors:  D W Slater; S Spiegelman
Journal:  Biophys J       Date:  2008-12-31       Impact factor: 4.033

3.  [Isolation and characterization of rapidly labelled high molecular RNA from freely suspended callus cells of parsley (Petroselinum sativum)].

Authors:  U Seitz; G Richter
Journal:  Planta       Date:  1970-12       Impact factor: 4.116

4.  [Isolation and characterization of rapidly labelled RNA from Euglena gracilis by means of analytical and preparative electrophoresis in polyacrylamide gels].

Authors:  E Spiess; G Richter
Journal:  Arch Mikrobiol       Date:  1970

5.  Partial characterization of transfer RNA genes isolated from Neurospora crassa.

Authors:  S K Dutta; R Ray
Journal:  Mol Gen Genet       Date:  1973-09-27

6.  Fractionation of native and denatured transforming deoxyribonucleic acid from Bacillus subtilis.

Authors:  S R Ayad; G R Barker; J Weigold
Journal:  Biochem J       Date:  1968-04       Impact factor: 3.857

7.  Methods for the analysis of deoxyribonucleic acid-ribonucleic acid hybridization data.

Authors:  J O Bishop; F W Robertson; J A Burns; M Melli
Journal:  Biochem J       Date:  1969-11       Impact factor: 3.857

8.  Recognition of size and sequence by an RNA replicase.

Authors:  I Haruna; S Spiegelman
Journal:  Proc Natl Acad Sci U S A       Date:  1965-10       Impact factor: 11.205

9.  A rationale for an analysis of RNA replication.

Authors:  S Spiegelman; I Haruna
Journal:  Proc Natl Acad Sci U S A       Date:  1966-06       Impact factor: 11.205

10.  Avidity of anti-DNA antibodies in serum and IgG glomerular eluates from patients with systemic lupus erythematosus. Association of high avidity antinative DNA antibody with glomerulonephritis.

Authors:  J B Winfield; I Faiferman; D Koffler
Journal:  J Clin Invest       Date:  1977-01       Impact factor: 14.808
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