Literature DB >> 10793757

Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease.

J N Hansen, B H Pheiffer, C J Hough.   

Abstract

A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.

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Substances：

Year:  1974        PMID： 10793757      PMCID： PMC343380          DOI： 10.1093/nar/1.6.787

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  CHROMATOGRAPHIC SEPARATION OF ANNEALED AND ENZYMATICALLY SYNTHESIZED RNA-DNA HYBRIDS.

Authors:  M N HAYASHI; M HAYASHI; S SPIEGELMAN
Journal:  Biophys J       Date:  1965-03       Impact factor: 4.033

2.  SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors:  F W STUDIER
Journal:  J Mol Biol       Date:  1965-02       Impact factor: 5.469

3.  Estimation of the double-helical content in various single-stranded nucleic acids by treatment with a single strand-specific nuclease.

Authors:  K Shishido; T Ando
Journal:  Biochim Biophys Acta       Date:  1972-12-22

4.  Use of exhaustive nucleic acid hybridization for determining the amount and size distribution of newly synthesized bacterial message RNA.

Authors:  D Gillespie; A Jacobson; S Gillespie; D Patterson
Journal:  Biochem Genet       Date:  1973-10       Impact factor: 1.890

5.  Isolation of a high-molecular-weight hybrid between ribosomal RNA and DNA.

Authors:  S Spadari; G Mazza; A Falaschi
Journal:  Eur J Biochem       Date:  1972-07-24

6.  Electron microscope mapping of the distribution of ribosomal genes of the Bacillus subtilis chromosome.

Authors:  L T Chow; N Davidson
Journal:  J Mol Biol       Date:  1973-04-05       Impact factor: 5.469

7.  Isolation of repetitive DNA from two mammalian species using S1 nuclease.

Authors:  M L Mace; R W Clark; F E Arrighi
Journal:  Biochem Biophys Res Commun       Date:  1974-01       Impact factor: 3.575

8.  Mung bean nuclease I. V. A tool for probing low melting regions in deoxyribonucleic acid.

Authors:  W Kedzierski; M Laskowski
Journal:  J Biol Chem       Date:  1973-02-25       Impact factor: 5.157

9.  Isolation of Escherichia coli transfer RNA-gene hybrids.

Authors:  A Marks; J H Spencer
Journal:  J Mol Biol       Date:  1970-07-14       Impact factor: 5.469

10.  Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.

Authors:  V M Vogt
Journal:  Eur J Biochem       Date:  1973-02-15
  10 in total
  3 in total

Review 1.  Expression profiling following traumatic brain injury: a review.

Authors:  Paolo G Marciano; James H Eberwine; Ramesh Ragupathi; Kathryn E Saatman; David F Meaney; Tracy K McIntosh
Journal:  Neurochem Res       Date:  2002-10       Impact factor: 3.996

2.  The specificity of S1 nuclease toward RNA-DNA hybrids as studied using isotopes of phosphorus-32 and phosphorus-33.

Authors:  S C Wittelsberger; J N Hansen
Journal:  Nucleic Acids Res       Date:  1977-06       Impact factor: 16.971

3.  Detection of AKR MuLV-specific RNA in AKR mouse cells by in situ hybridization.

Authors:  C Godard; K W Jones
Journal:  Nucleic Acids Res       Date:  1979-06-25       Impact factor: 16.971
  3 in total

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