Literature DB >> 14268724

ENZYMATIC SYNTHESIS OF TRI- AND TETRANUCLEOTIDES OF DEFINED SEQUENCE.

R E THACH, P DOTY.   

Abstract

Conditions have been found under which polynucleotide phosphorylase adds only a few nucleotide residues to the 3' end of a dinucleotide primer. Pure tri- and tetranucleotides can be isolated from reaction mixtures in high yield.

Entities:  

Keywords:  CARBON ISOTOPES; CHEMISTRY; CHROMATOGRAPHY; EXPERIMENTAL LAB STUDY; NUCLEOTIDYLTRANSFERASES; POLYNUCLEOTIDES; RADIOMETRY

Mesh:

Substances:

Year:  1965        PMID: 14268724     DOI: 10.1126/science.148.3670.632

Source DB:  PubMed          Journal:  Science        ISSN: 0036-8075            Impact factor:   47.728


  9 in total

1.  Thermodynamic and kinetic properties of short RNA helices: the oligomer sequence AnGCUn.

Authors:  J Ravetch; J Gralla; D M Crothers
Journal:  Nucleic Acids Res       Date:  1974-01       Impact factor: 16.971

2.  Isoaccepting species of serine tRNA coded by bacteriophage T5sto.

Authors:  G Henckes; O Panayotakis; T Heyman
Journal:  J Virol       Date:  1976-02       Impact factor: 5.103

3.  On the mechanism of oligonucleotide-primed RNA synthesis. I. Model studies with deoxyhomopolymer templates and Escherichia coli RNA polymerase.

Authors:  C F Van Kreijl; R H Beelen; P Borst
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

4.  Ribonucleic acid synthesis by spermatozoa from the rat and hamster.

Authors:  J MacLaughlin; C Terner
Journal:  Biochem J       Date:  1973-08       Impact factor: 3.857

5.  Loss of coding properties of the oligonucleotide adenylyluridylylguanosine after photoreduction or hydration.

Authors:  S Lee; G L Brown; Z Kosinski
Journal:  Biochem J       Date:  1967-06       Impact factor: 3.857

6.  RNA codewords and protein synthesis, 8. Nucleotide sequences of synonym codons for arginine, valine, cysteine, and alanine.

Authors:  R Brimacombe; J Trupin; M Nirenberg; P Leder; M Bernfield; T Jaouni
Journal:  Proc Natl Acad Sci U S A       Date:  1965-09       Impact factor: 11.205

7.  The polarity of messenger translation in protein synthesis.

Authors:  R E Thach; M A Cecere; T A Sundararajan; P Doty
Journal:  Proc Natl Acad Sci U S A       Date:  1965-10       Impact factor: 11.205

8.  Polynucleotides. XLII1. Limited addition of 2'O-onitrobenzyl nucleotides to the 3'-end of ribooligonucleotide with polynucleotide phosphorylase.

Authors:  M Ikehara; S Tanaka; T Fukui; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1976-11       Impact factor: 16.971

9.  Fluorine-19 nuclear magnetic resonance study of codon-anticodon interaction in 5-fluorouracil-substituted E. coli transfer RNAs.

Authors:  P Gollnick; C C Hardin; J Horowitz
Journal:  Nucleic Acids Res       Date:  1986-06-11       Impact factor: 16.971

  9 in total

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