| Literature DB >> 14258729 |
P E DUNAGIN, E H MEADOWS, J A OLSON.
Abstract
The major metabolite in rat bile of injected C(14)-retinoic acid was purified by ion-exchange and silicic acid chromatography; it has the spectrum of methyl retinoate, releases retinoic acid upon basic hydrolysis or by treatment with beta-glucuronidase, and contains glucuronic acid. The metabolite was characterized by treatment with diazomethane followed by hexamethyl-disilazane, or with periodate followed by semicarbazide, and the products were chromatographed. The metabolite has been tentatively identified as retinoyl beta-glucuronide.Entities:
Keywords: BILE; CHROMATOGRAPHY; ENZYME INHIBITORS; EXPERIMENTAL LAB STUDY; GLUCURONATES; GLUCURONIDASE; LACTONES; METABOLISM; PHENOLPHTHALEINS; RATS; VITAMIN A
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Year: 1965 PMID： 14258729 DOI： 10.1126/science.148.3666.86
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728