CELL-CULTURE RESPONSE TO FIBROMA VIRUS.

Verna, John E. (University of Minnesota, Minneapolis). Cell-culture response to fibroma virus. J. Bacteriol. 89:524-528. 1965.-Three established rabbit cell lines (Minnesota CRP, RbK, and RbH) were infected with Shope's rabbit fibroma virus (Patuxent strain). The responses of the cell lines to virus infection differed from one another. Minn. CRP cells produced plaques (1 to 2 mm) on the 4th or 5th day which possessed characteristic cellular aggregates at the periphery of the plaque. Minn. RbH cells underwent degeneration with the elongation of cells and no aggregation formation. Minn. RbK cells responded with foci of cellular aggregates and no plaque formation. The resulting number of characteristic lesions in each cell line was related to virus dilution. The history of virus passage had no effect on the resulting lesion. All cells appeared to be equally sensitive to fibroma virus infection. Conclusive evidence for a viral-induced proliferative effect in RbK cells has not been found.