| Literature DB >> 1425671 |
T Watanabe1, T Sato, S Yoshioka, T Koshijima, M Kuwahara.
Abstract
Beta-glucosidase was purified from a crude cellulase preparation from Aspergillus niger by affinity chromatography on a methacrylamide-N-methylene-bis-methacrylamide copolymer bearing cellobiamine. The purified enzyme was a dimer with an isoelectric point of 4.0. The molecular mass of the enzyme was estimated to be 240 kDa by gel-permeation chromatography. The enzyme hydrolyzed specifically beta-glucosidic bonds and catalyzed transglucosylation of the beta-glucosyl group of cellobiose to yield 4-O-beta-gentiobiosylglucose in the presence of organic solvents or under neutral conditions.Entities:
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Year: 1992 PMID： 1425671 DOI： 10.1111/j.1432-1033.1992.tb17332.x
Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956