| Literature DB >> 14234776 |
T SUZUKI, E A GORZYNSKI, E NETER.
Abstract
Suzuki, T. (Children's Hospital, Buffalo, N.Y.), E. A. Gorzynski, and E. Neter. Separation by ethanol of common and somatic antigens of Enterobacteriaceae. J. Bacteriol. 88:1240-1243. 1964.-Ethanol in a concentration of 85% permits separation from crude culture supernatant fluids of the common enterobacterial antigen and the O antigen, the former being ethanol-soluble, the latter being ethanol-insoluble. The evidence was obtained in hemagglutination, hemolysis, and hemagglutination-inhibition tests. In contrast to the crude antigens obtained from enteric bacteria other than Escherichia coli O14, the ethanol-soluble fraction is immunogenic in the rabbit upon intravenous injection, and antibodies against the common antigen are produced in relatively high titers. Aqueous mixtures of ethanol-soluble and -insoluble fractions engender antibodies against the common antigen in titers significantly lower than those stimulated by the soluble fraction alone. Ethanol-treated whole antigen fails to stimulate antibody formation against this antigen. These results suggest the presence of an inhibitor in the ethanol-insoluble fraction.Entities:
Keywords: ALCOHOL, ETHYL; ANTIBODY FORMATION; ANTIGENS; BACTERIOLOGICAL TECHNICS; ENTEROBACTERIACEAE; EXPERIMENTAL LAB STUDY; HEMAGGLUTINATION; HEMAGGLUTINATION INHIBITION TESTS; HEMOLYSIS; RABBITS
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Year: 1964 PMID： 14234776 PMCID： PMC277399 DOI： 10.1128/jb.88.5.1240-1243.1964
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490