Literature DB >> 14227439

ESTIMATION OF SERUM HAEMOGLOBIN-BINDING CAPACITY (HAPTOGLOBIN) ON SEPHADEX G.100.

A P RATCLIFF, J HARDWICKE.   

Abstract

A method is described for the estimation of serum haptoglobin as ;haemoglobin-binding capacity'. The method relies on column chromatographic separation of the haemoglobin/haptoglobin complex from excess added free haemoglobin on the dextran gel Sephadex G.100. The method is simple and reproducible, and correlates well with another method of estimating haemoglobin-binding capacity over a wide range of values.

Entities:  

Keywords:  CHROMATOGRAPHY; DEXTRAN; HAPTOGLOBINS; HEMOGLOBIN; IMMUNOELECTROPHORESIS

Mesh：

Substances：

Year:  1964        PMID： 14227439      PMCID： PMC480856          DOI： 10.1136/jcp.17.6.676

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  7 in total

1.  Methaemalbumin in the differential diagnosis of acute haemorrhagic and oedematous pancreatitis.

Authors:  B E NORTHAM; D S ROWE; N E WINSTONE
Journal:  Lancet       Date:  1963-02-16       Impact factor: 79.321

2.  A rapid method for the estimation of serum haptoglobin.

Authors:  D S ROWE
Journal:  J Clin Pathol       Date:  1961-03       Impact factor: 3.411

3.  Human haptoglobins: estimation and purification.

Authors:  G E CONNELL; O SMITHIES
Journal:  Biochem J       Date:  1959-05       Impact factor: 3.857

4.  The binding of haematin by serum proteins.

Authors:  G M ABER; D S ROWE
Journal:  Br J Haematol       Date:  1960-04       Impact factor: 6.998

5.  Studies on the serum haptoglobin level in hemoglobinemia and its influence on renal excretion of hemoglobin.

Authors:  C B LAURELL; M NYMAN
Journal:  Blood       Date:  1957-06       Impact factor: 22.113

6.  The demonstration of intravascular haemolysis by means of serum paper electrophoresis and a modification of Schumm's reaction.

Authors:  F C NEALE; G M ABER; B E NORTHAM
Journal:  J Clin Pathol       Date:  1958-05       Impact factor: 3.411

7.  [Method for the determination of serum haptoglobin].

Authors:  M F JAYLE
Journal:  Bull Soc Chim Biol (Paris)       Date:  1951
  7 in total
  10 in total

1.  Gamma glutamyl transpeptidase levels in arthritis: a correlation with clinical and laboratory indices of disease activity.

Authors:  J R Lowe; M E Pickup; J S Dixon; P A Leatham; V M Rhind; V Wright; W W Downie
Journal:  Ann Rheum Dis       Date:  1978-10       Impact factor: 19.103

2.  The measurement of renal permeability using labelled marcromolecules.

Authors:  B Hulme; J Hardwicke
Journal:  Proc R Soc Med       Date:  1966-06

3.  Effects of gold, dapsone, and prednisone on serum C-reactive protein and haptoglobin and the erythrocyte sedimentation rate in rheumatoid arthritis.

Authors:  B McConkey; P Davies; R A Crockson; A P Crockson; M Butler; T J Constable; R S Amos
Journal:  Ann Rheum Dis       Date:  1979-04       Impact factor: 19.103

4.  Incorporation of 14C-thymidine by cultures of erythrocytes from rheumatoid arthritis patients and normal subjects, suggesting the presence of an L-form.

Authors:  P E Pease; R Bartlett; M Farr
Journal:  Experientia       Date:  1981-05-15

5.  Inhibition by alpha-macroglobulin and other serum proteins.

Authors:  P M Starkey; A J Barrett
Journal:  Biochem J       Date:  1973-04       Impact factor: 3.857

6.  Effect of oral D-penicillamine treatment on experimental arthritis and associated immune responses in rabbits. III: Reduction of the monoarticular arthritis.

Authors:  I M Hunneyball; G A Stewart; D R Stanworth
Journal:  Ann Rheum Dis       Date:  1979-06       Impact factor: 19.103

7.  Discriminatory indices of response of patients with rheumatoid arthritis treated with D-penicillamine.

Authors:  J S Dixon; M E Pickup; J R Lowe; C Hallett; M R Lee; V Wright
Journal:  Ann Rheum Dis       Date:  1980-08       Impact factor: 19.103

8.  Alpha-2-macroglobulin levels in disease in man.

Authors:  J Housley
Journal:  J Clin Pathol       Date:  1968-01       Impact factor: 3.411

9.  Detection by electron microscope of rod-shaped organisms in synovial membrane from a patient with the arthritis of Whipple's disease.

Authors:  C F Hawkins; M Farr; C J Morris; A M Hoare; N Williamson
Journal:  Ann Rheum Dis       Date:  1976-12       Impact factor: 19.103

10.  Rheumatoid arthritis: effects of a new agent (ICI 55 897) on serum acute phase proteins and the erythrocyte sedimentation rate.

Authors:  B McConkey; R S Amos; M E Billingham; T J Constable; R A Crockson; A P Crockson; P J Forster
Journal:  Ann Rheum Dis       Date:  1980-02       Impact factor: 19.103
  10 in total

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