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Literature DB >> 14212122

EQUINE ANTIHAPTEN ANTIBODY. I. 7S BETA-2A- AND 1OS GAMMA-1- GLOBULIN COMPONENTS OF PURIFIED ANTI-BETA-LACTOSIDE ANTIBODY.

Abstract

Anti-p-azophenyl-beta-lactoside (Lac) antibody has been isolated from equine antiserum by specific precipitation with Lac-HSA (human serum albumin) and Lac-Hy (hemocyanin). Antibody was separated from antigen by chromatography on DEAE cellulose and zone electrophoresis in solvents containing lactose. Six antigenically distinct immunoglobulins have been identified in purified equine anti-Lac antibody: 7S beta(2A)-globulin, 19S gamma-globulin, a 10S gamma(1) globulin, and three antigenically distinct 7S gamma-globulins. The specific hapten-binding activity of the 7S beta(2A)-antibody and of the 10S gamma(1)-antibody has been demonstrated by equilibrium dialysis and by analytical ultracentrifugation using a combination of schlieren and absorption optics. The 10S gamma(1)-globulin antibody may be representative of a hitherto undescribed class of immunoglobulins.

Entities: Chemical Gene Species

Keywords: ANTIBODIES; BETA GLOBULIN; CHROMATOGRAPHY; EXPERIMENTAL LAB STUDY; GAMMA GLOBULIN, 7S; HAPTENS; HORSES; IMMUNOELECTROPHORESIS; LACTOSE; SERUM ALBUMIN; ULTRACENTRIFUGATION

Mesh：

Substances：

Year: 1964 PMID： 14212122 PMCID： PMC2137781 DOI： 10.1084/jem.120.4.589

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

31 in total

1. IMMUNOELECTROPHORETIC CHARACTERIZATION OF HUMAN SERUM PROTEINS WITH PRIMATE ANTISERA.

Authors: E A LICHTER; S DRAY
Journal: J Immunol Date: 1964-01 Impact factor: 5.422

2. On structural and functional relations between antibodies and proteins of the gamma-system.

Authors: G M EDELMAN; B BENACERRAF
Journal: Proc Natl Acad Sci U S A Date: 1962-06-15 Impact factor: 11.205

3. Ultracentrifugal characterization of antibodies in sera of ragweed-sensitive individuals.

Authors: L GYENES; J GORDON; A H SEHON
Journal: Immunology Date: 1961-04 Impact factor: 7.397

4. Thermodynamic study of the isohaemagglutinins.

Authors: R WURMSER; S FILITTI-WURMSER
Journal: Prog Biophys Biophys Chem Date: 1957

5. Properties of guinea pig 7S antibodies. II. Identification of antibodies involved in passive cutaneous and systemic anaphylaxis.

Authors: Z OVARY; B BENACERRAF; K J BLOCH
Journal: J Exp Med Date: 1963-06-01 Impact factor: 14.307

6. TWO MAJOR TYPES OF NORMAL 7S gamma-GLOBULIN.

Authors: M Mannik; H G Kunkel
Journal: J Exp Med Date: 1963-01-31 Impact factor: 14.307

7. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies.

Authors: B BENACERRAF; Z OVARY; K J BLOCH; E C FRANKLIN
Journal: J Exp Med Date: 1963-06-01 Impact factor: 14.307

8. CLASSIFICATION OF MYELOMA PROTEINS, BENCE JONES PROTEINS, AND MACROGLOBULINS INTO TWO GROUPS ON THE BASIS OF COMMON ANTIGENIC CHARACTERS.

Authors: M Mannik; H G Kunkel
Journal: J Exp Med Date: 1962-11-30 Impact factor: 14.307

9. THE MOLECULAR WEIGHT OF ANTIBODIES.

Authors: E A Kabat
Journal: J Exp Med Date: 1939-01-01 Impact factor: 14.307

10. DIFFERENCES IN THE ELECTROPHORETIC MOBILITIES OF GUINEA PIG 7S ANTIBODIES OF DIFFERENT SPECIFICITIES.

Authors: V NUSSENZWEIG; B BENACERRAF
Journal: J Exp Med Date: 1964-03-01 Impact factor: 14.307

12 in total

1. Production of antibodies of identical idiotype but diverse immunoglobulin classes by cells derived from a single stimulated B cell.

Authors: P J Gearhart; N H Sigal; N R Klinman
Journal: Proc Natl Acad Sci U S A Date: 1975-05 Impact factor: 11.205

2. The antigenic and immunosuppressive properties of normal and antilymphocytic equine IgG subfractions.

Authors: R A Allardyce; N F Anderson; J P Vaerman; K James
Journal: Clin Exp Immunol Date: 1973-02 Impact factor: 4.330

3. Immunoglobulins of mink. Evidence for five immunoglobulin classes of 7S type.

Authors: H Tabel; D G Ingram
Journal: Immunology Date: 1972-06 Impact factor: 7.397

4. Identification and quantitation of equine serum and secretory immunoglobulin A.

Authors: T C McGuire; T B Crawford
Journal: Infect Immun Date: 1972-10 Impact factor: 3.441

5. Foot-and-mouth disease in swine. II. Some physical-chemical characteristics of antibodies produced by chemically-treated and non-treated foot-and-mouth disease virus.

Authors: P D McKercher; A R Giordano
Journal: Arch Gesamte Virusforsch Date: 1967

6. Studies on the IgA system of the horse.

Authors: J P Vaerman; P Querinjean; J F Heremans
Journal: Immunology Date: 1971-09 Impact factor: 7.397

7. Analysis of the biological activity of antilymphocyte serum. 3. Concentration of immunosuppressive activity in one IgG subfraction and its inhibition by another.

Authors: R J Perper; R E Monovich; T J Van Gorder
Journal: Immunology Date: 1971-12 Impact factor: 7.397