GROWTH OF THIOBACILLUS THIOOXIDANS IN SHAKEN CULTURE.

Cook, Thomas M. (Rutgers, The State University, New Brunswick, N.J.). Growth of Thiobacillus thiooxidans in shaken culture. J. Bacteriol. 88:620-623. 1964.-When flasks of sulfur-containing medium were inoculated with Thiobacillus thiooxidans and placed on a shaking machine immediately after inoculation, growth was markedly inhibited. If various wetting agents were provided, or if the ratio of inoculum to sulfur was increased, growth under shaking conditions was possible. Evidence cited shows that the natural wetting agent (or other externally supplied surfactants) is necessary to effect a bacteria-sulfur contact which is stable to shaking conditions.