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Literature DB >> 14197875

DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII.

M E TOURTELLOTTE, H J MOROWITZ, P KASIMER.

Abstract

Tourtellotte, Mark E. (University of Connecticut, Storrs), Harold J. Morowitz, and Phil Kasimer. Defined medium for Mycoplasma laidlawii. J. Bacteriol. 88:11-15. 1964.-A defined medium for the pleuropneumonia-like organism Mycoplasma laidlawii B is described in which absolute requirements for coenzyme A and longchain fatty acids were demonstrated. This organism did not require cholesterol or macromolecules of high molecular weight, but did show a growth requirement for peptides. Optimal growth in the basal medium was obtained in the presence of two purified peptides from crystalline ribonuclease, one of which has the amino acid sequence threonine - threonine - glutamine - alanine - asparagine-lysine, and the other lysine-glutamic acid-threonine-alanine-alanine-alanine-lysine. Continuous, but suboptimal, growth was obtained with the single ribonuclease peptide: lysine-glutamic acid-threonine-alanine-alanine-alanine-lysine.

Entities: Chemical Disease Species

Keywords: CULTURE MEDIA; EXPERIMENTAL LAB STUDY; MYCOPLASMA

Mesh：

Substances：

Year: 1964 PMID： 14197875 PMCID： PMC277248 DOI： 10.1128/jb.88.1.11-15.1964

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

11 in total

2. Isolation of Acholeplasma laidlawii from commercial, serum-free tissue culture medium and studies on its survival and detection.

Authors: I E Low
Journal: Appl Microbiol Date: 1974-06

DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII.

1. Synthetic media for pleuropneumonialike organisms.

2. Nutritional requirements and metabolism of Mycoplasma laidlawii.

3. Nutritional requirements of PPLO and their relation to metabolic function.

4. The structure of ribonuclease.

5. Enumeration of pleuropneumonia-like cultures.

6. Nutrition and metabolism of Mycoplasma mycoides var. mycoides.

7. Peptides and bacterial growth. VIII. The nature of strepogenin.

8. The chemical composition and submicroscopic morphology of Mycoplasma gallisepticum, avian PPLO 5969.

9. LIPID COMPOSITION AND SYNTHESIS IN THE PLEUROPNEUMONIA-LIKE ORGANISM MYCOPLASMA GALLISEPTICUM.

10. Peptides required for the growth of Lactobacillus bulgaricus.

Review 1. Regulating the Intersection of Metabolism and Pathogenesis in Gram-positive Bacteria.

2. Isolation of Acholeplasma laidlawii from commercial, serum-free tissue culture medium and studies on its survival and detection.

3. Requirements of Acholeplasma laidlawii A, strain LA 1, for nucleic acid precursors.

4. Use of dialyzing culture technique for high yield of Mycoplasma.

5. Comparative biosynthesis of ornithine and lysine by Mycoplasma and L forms.

6. Synthesis of deoxyribomononucleotides in Mollicutes: dependence on deoxyribose-1-phosphate and PPi.

7. Synthesis of saturated long chain fatty acids from sodium acetate-1-C14 by Mycoplasma.

8. Physiological and serological comparisons among strains of Mycoplasma granularum and Mycoplasma laidlawii.

9. Uracil phosphoribosyltransferase from Acholeplasma laidlawii: partial purification and kinetic properties.

10. Characteristics of a new sterol-nonrequiring Mycoplasma.