| Literature DB >> 14176290 |
Abstract
It was previously reported from this laboratory that the secondary antibody response can regularly be elicited in vitro from fragments of rabbit lymph node node cultured in Eagle's medium supplemented with normal rabbit serum. Evidence is now presented that physiological levels of hydrocortisone (0.01 to 1.0 microM) can substitute for serum in the culture medium. However, with the omission of serum, serine (0.1 mM) must be included among Eagle's "essential" amino acids for consistent optimal antibody production. In some experiments the addition of insulin (0.5 unit/ml) and vitamin B(12) (0.5 microg/ml) has further enhanced the secondary response in this serum-free medium.Entities:
Keywords: ADRENAL CORTEX HORMONES; AMINO ACIDS; ANTIBODY FORMATION; CULTURE MEDIA; EXPERIMENTAL LAB STUDY; HYDROCORTISONE; INSULIN; RABBITS; SUCCINATES; TISSUE CULTURE; TRIIODOTHYRONINE; VITAMIN B 12; VITAMINS
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Year: 1964 PMID： 14176290 PMCID： PMC2137758 DOI： 10.1084/jem.119.6.1027
Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307