Literature DB >> 28078501

The Tissue Culture Laboratory of Dr. George Otto Gey 60 yrs ago as recalled by a former student.

Charles T Ambrose1.   

Abstract

George Otto Gey was a pioneer in tissue culture, having introduced the roller drum, the HeLa cell line, and the use of human fetal cord serum and beef embryo extract. During his career (1920s-1960s), the field of tissue culture was in its infancy and not yet dependent upon commercial biological supply houses. While the early techniques of cell culture have been greatly improved upon, of historical interest may be personal observations of the Gey Tissue Culture Laboratory, Johns Hopkins Medical School, as recalled by a medical student working there in the 1950s. Dr. Gey served as a founding member and executive of the Tissue Culture Commission (TCC) and became the first president of the Tissue Culture Association (TCA).

Entities:  

Keywords:  Antibody production; George Gey; Hopkins medical school; Margaret Gey; Tissue culture

Mesh:

Year:  2017        PMID: 28078501     DOI: 10.1007/s11626-016-0128-8

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol Anim        ISSN: 1071-2690            Impact factor:   2.416


  16 in total

1.  Antibody production in organ cultures of lymph-node fragments following in vitro secondary antigenic stimulation.

Authors:  C T AMBROSE
Journal:  Natl Cancer Inst Monogr       Date:  1963-03

2.  The effect of calcium and magnesium on the growth and morphology of mouse lymphoblasts (MB II, de Bruyn) in tissue culture.

Authors:  O V OWENS; M K GEY; G O GEY
Journal:  Cancer Res       Date:  1958-09       Impact factor: 12.701

3.  Robert Koch.

Authors:  L Brown
Journal:  Bull N Y Acad Med       Date:  1932-09

4.  [A matter of methods: the historicity of Koch's postulates 1840-2000].

Authors:  Christoph Gradmann
Journal:  Medizinhist J       Date:  2008

5.  EFFECT OF THE AMINO ACIDS AND DIALYZABLE CONSTITUENTS OF EMBRYONIC TISSUE JUICE ON THE GROWTH OF FIBROBLASTS.

Authors:  L E Baker; A Carrel
Journal:  J Exp Med       Date:  1926-08-31       Impact factor: 14.307

6.  Serum-free cell culture: a unifying approach.

Authors:  D Barnes; G Sato
Journal:  Cell       Date:  1980-12       Impact factor: 41.582

7.  George Otto Gey. (1899-1970). The HeLa cell and a reappraisal of its origin.

Authors:  H W Jones; V A McKusick; P S Harper; K D Wuu
Journal:  Obstet Gynecol       Date:  1971-12       Impact factor: 7.661

8.  STUDIES UPON THE CHARACTERISTICS OF DIFFERENT CULTURE MEDIA AND THEIR INFLUENCE UPON THE GROWTH OF TISSUE OUTSIDE OF THE ORGANISM.

Authors:  R Ingebrigtsen
Journal:  J Exp Med       Date:  1912-10-01       Impact factor: 14.307

9.  THE PRODUCTION OF ANTIBODIES BY TISSUES LIVING OUTSIDE OF THE ORGANISM.

Authors:  A Carrel; R Ingebrigtsen
Journal:  J Exp Med       Date:  1912-03-01       Impact factor: 14.307

10.  ANTAGONISTIC GROWTH PRINCIPLES OF SERUM AND THEIR RELATION TO OLD AGE.

Authors:  A Carrel; A H Ebeling
Journal:  J Exp Med       Date:  1923-09-30       Impact factor: 14.307

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